Monoclonal antibodies to the carboxy-terminal Ea sequence of pro-insulin-like growth factor-IA (proIGF-IA) recognize proIGF-IA secreted by IM9 B-lymphocytes

Growth Hormone and IGF Research

Volumn 11, Issue 1, 2001, Pages 10-17

  Wilson, H E ^a   Westwood, M ^a   White, A ^a,b   Clayton, P E ^a  

^a Endocrine Sciences Research Group   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Ea peptide; Glycosylation; Insulin like growth factors; Monoclonal antibodies; Pro hormones; Pro IGF IA

Indexed keywords

COMPLEMENTARY DNA; GLYCOSIDASE; HORMONE PRECURSOR; MONOCLONAL ANTIBODY; PEPTIDE E; PROINSULIN LIKE GROWTH FACTOR IA; PROINSULIN LIKE GROWTH FACTOR IB; SOMATOMEDIN C; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; B LYMPHOCYTE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; EMBRYO; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LYMPHOID CELL LINE; MOLECULAR RECOGNITION; PREDICTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; SERUM;

EID: 0034752201     PISSN: 10966374     EISSN: None     Source Type: Journal    
DOI: 10.1054/ghir.2000.0182     Document Type: Article

References (34)

1. Role of insulin-like growth factors in embryonic and postnatal growth
2. Intrauterine growth retardation and postnatal growth failure associated with deletion of the insulin-like growth factor-I gene
3. Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations
4. A mitogenic peptide amide encoded within the E peptide domain of the insulin-like growth factor IB prohormone
5. Recombinant E-peptides of Pro-IGF-I have mitogenic activity
6. Alveolar macrophages release an insulin-like growth factor I-type molecule
7. Release of somatomedin-like activity by cultured WI-38 human fibroblasts
8. Human hepatoma cells synthesize and secrete insulin-like growth factor Ia prohormone under growth hormone control
9. Expression of the genes encoding the insulin-like growth factors and their receptors in the human ovary
10. Expression of insulin-like growth factor-IA and factor-IB mRNA in human liver, hepatoma cells, macrophage-like cells and fibroblasts
11. Cultured fibroblasts secrete insulin-like growth factor IA prohormone
12. Antiserum developed for the E peptide region of insulin-like growth factor IA prohormone recognises a serum protein by both immunoblot and radioimmunoassay
13. Production of monoclonal antibodies: Strategy and tactics
14. Measurement of insulin-like growth factor-II in human plasma using a specific monoclonal antibody-based two-site immunoradiometric assay
15. Elevated plasma insulin-like growth factor binding protein-1 levels in type 1 (insulin-dependent) diabetic patients with peripheral neuropathy
16. Mutational analysis of the insulin-like growth factor I prohormone processing site
17. Introduction of DNA into mammalian cells
18. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
19. Growth hormone induces tyrosine phosphorylation but does not alter insulin-like growth factor-I gene expression in human IM9 lymphocytes
20. Obesity and impaired prohormone processing associated with mutations in the human prohormone convertase 1 gene
21. Carboxypeptidase E is a regulated secretory pathway sorting receptor: Genetic obliteration leads to endocrine disorders in Cpe (fat) mice
22. Does C-peptide have a physiological role?
23. Prevention of vascular and neural dysfunction in diabetic rats by c-peptide
24. Obesity, diabetes and functions for proopiomelanocortin-derived peptides
25. Extraction and nutritional/hormonal regulation of tissue insulin-like growth factor-I activity
26. Biosynthesis of human insulin-like growth factor I (IGF-I). The primary translation product of IGF-I mRNA contains an unusual 48-amino acid signal peptide
27. Alternative splicing produces messenger RNAs encoding insulin-like growth factor-I prohormones that are differentially glycosylated in vitro
28. Role of glycosylaiton in the processing of newly translated insulin proreceptor in 3T3-L1 adipocytes
29. Characterization of fibroblast growth factor-6 expressed by Chinese hamster ovary cells as a glycosylated mitrogen for human vascular endothelial cells
30. Recombinant human epidermal growth factor precursor is a glycosylated membrane protein with biological activity
31. The TGF-alpha precursor expressed on the cell surface binds to the EGF receptor on adjacent cells, leading to signal transduction
32. Differential regulation of transcription initiation from insulin-like growth factor-I (IGF-I) leader exons and of tissue IGF-I expression in response to changed growth hormone and nutritional status in sheep
33. Processing of wild-type and mutant proinsulin-like growth factor-IA by subtilisin-related proprotein convertases
34. Positional and additive effects of basic amino acids on processing of precursor proteins within the constitutive secretory pathway