Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals

Brazilian Journal of Medical and Biological Research

Volumn 34, Issue 10, 2001, Pages 1271-1275

  Barabas E ^a   Nagy, K ^a   Varkonyi V ^a   Horvath A ^a  

^a Natl Inst Dermatol and Venerology   (Hungary)

Author keywords

HIV; IFN mRNA; Quantitative competitive RT PCR; Semiquantitative RT PCR

Indexed keywords

CD4 ANTIGEN; CYTOKINE; GAMMA INTERFERON; MESSENGER RNA;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ARTICLE; CELL STIMULATION; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION FUNCTION; EVALUATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; LYMPHOCYTE COUNT; MONONUCLEAR CELL; QUANTITATIVE ASSAY; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGY; STANDARD; T LYMPHOCYTE;

HUMAN IMMUNODEFICIENCY VIRUS;

EID: 0034749340     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2001001000006     Document Type: Article

References (12)

1. A Th1-Th2 switch is a critical step in the etiology of HIV infection
2. Expression of IL-10 and IL-4 and interferon-gamma in unstimulated and mitogen stimulated peripheral blood lymphocytes from HIV-seropositive patients
3. TH1 to TH2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions
4. Elevated IFN-γ and decreased IL-2 gene expression are associated with HIV infection
5. Lack of evidence for the dichotomy of Th1 and Th2 predominance in HIV-infected individuals
6. Cytokine gene expression in HIV-infected intestinal mucosa
7. Circulating CD8 T cells show increased interferon-γ mRNA expression in HIV infection
8. The dominant T-helper lymphocyte function of HIV infected patients
9. Atopy, anergic status, and cytokine expression in HIV-infected subjects
10. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
11. An improved general method to generate internal standards for competitive PCR