Second vidal coefficients as a measure of protein-osmolyte interactions

Protein Science

Volumn 10, Issue 1, 2001, Pages 12-16

  Weatherly, G T ^a   Pielak, G J ^a  

^a University of North Carolina at Chapel Hill   (United States)

Author keywords

Analytical ultracentrifugation; Cytochrome c; Excluded volume; Osmolytes; Second virial coefficient

Indexed keywords

CYTOCHROME C; PROTEIN; OLIGOSACCHARIDE; SERUM ALBUMIN; STACHYOSE;

ARTICLE; CYTOPLASM; DENSITY GRADIENT CENTRIFUGATION; MACROMOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STABILITY; ANIMAL; CATTLE; ESCHERICHIA COLI; METABOLISM; METHODOLOGY; SACCHAROMYCES CEREVISIAE; ULTRACENTRIFUGATION;

ANIMALS; CATTLE; CYTOCHROME C GROUP; ESCHERICHIA COLI; OLIGOSACCHARIDES; SACCHAROMYCES CEREVISIAE; SERUM ALBUMIN; ULTRACENTRIFUGATION;

EID: 0034745484     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.29301     Document Type: Article

References (24)

1. Oxidation state-dependent conformational changes in cytochrome c
2. Vapor pressure osmometry studies of osmolyte-protein interactions: Implications for the action of osmoprotectants in vivo and for the interpretation of "osmotic stress" experiments in vitro
3. Replacement of cysteine-107 of Saccharomyces cerevisiae iso-1-cytochrome c with threonine: Improved stability of the mutant protein
4. Sugar-induced molten-globule model
5. Specific volumes of biological macromolecules and other molecules of biological interest
6. Probing protein-sugar interactions
7. Molecular volumes and the Stokes-Einstein equation
8. Proton nuclear magnetic resonance as a probe of differences in structure between the C102T and F82S, C102T variants of iso-1-cytochrome c from the yeast Saccharomyces cerevisiae
9. On the understanding of the effect of salts. Second report. On regularities in the precipitating effect of salts and their relationship to their physiological behavior
10. Thermodynamic analysis of the effects of small inert cosolutes in the ultracentrifugation of noninteracting proteins
11. Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques
12. Sedimentation equilibrium as thermodynamic tool
13. 551
14. 15N-labeled eukaryotic cytochrome c in Escherichia coli
15. Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition
16. Osmolyte-induced changes in protein conformational equilibria
17. The hydration, size and shape of tobacco mosaic virus
18. Multiple effects of trehalose on protein folding in vitro and in vivo
19. Analysis of sedimentation equilibrium distributions reflecting nonideal macromolecular associations
20. Thermodynamic nonideality and protein solvation
21. The thermodynamic mechanism of protein stabilization by trehalose
22. Living with water stress: Evolution of osmolyte systems
23. Macromolecular crowding: Biochemical, biophysical, and physiological consequences