Superactivation of integrin αvβ3 by low antagonist concentrations

Journal of Cell Science

Volumn 114, Issue 8, 2001, Pages 1545-1553

  Legler, Daniel F ^a   Wiedle, Guido ^b   Patrick Ross, F ^c   Imhof, Beat A ^b  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^c BARNES JEWISH HOSPITAL   (United States)

Author keywords

Affinity modulation; Cyclo RGD; Integrin v 3; Intgrin activation; Soluble recombinant cell adhesion molecule; Vitronectin receptor

Indexed keywords

ARGINYLGLYCYLASPARTIC ACID; CALCIUM; CHIMERIC PROTEIN; DIVALENT CATION; FIBRINOGEN; FIBRONECTIN; INTEGRIN; INTEGRIN RECEPTOR; LIGAND; MAGNESIUM; MANGANESE; MEMBRANE PROTEIN; PROTEIN KISS 31; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VITRONECTIN; VITRONECTIN RECEPTOR; ARGINYL-GLYCYL-ASPARTIC ACID; OLIGOPEPTIDE;

AGONIST; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL MIGRATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HOMEOSTASIS; LIGAND BINDING; MAMMAL; MODULATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; RECEPTOR AFFINITY; SOLUBILITY; BIOLOGICAL MODEL; BIOSYNTHESIS; CELL LINE; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; DRUG POTENTIATION; GENETICS; HUMAN;

ANIMALIA; MAMMALIA;

CELL ADHESION; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; MODELS, BIOLOGICAL; OLIGOPEPTIDES; RECEPTORS, VITRONECTIN; RECOMBINANT PROTEINS;

EID: 0034744186     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Aumailley, M., Gurrath, M., Muller, G., Calvete, J., Timpl, R. and Kessler, H. (1991). Arg-Gly-Asp constrained within cyclic pentapeptides. Strong and selective inhibitors of cell adhesion to vitronectin and laminin fragment P1. FEBS Lett. 291, 50-54.
2. Bazzoni, G., Shih, D. T., Buck, C. A. and Hemler, M. E. (1995). Monoclonal antibody 9EG7 defines a novel beta 1 integrin epitope induced by soluble ligand and manganese, but inhibited by calcium. J. Biol. Chem. 270, 25570-25577.
3. Bazzoni, G. and Hemler, M. E. (1998). Are changes in integrin affinity and conformation overemphasized? Trends Biochem. Sci. 23, 30-34.
4. Bennett, J. S., Chan, C., Vilaire, G., Mousa, S. A. and DeGrado, W. F. (1997). Agonist-activated alphavbeta3 on platelets and lymphocytes binds to the matrix protein osteopontin. J. Biol. Chem. 272, 8137-8140.
5. Bergelson, J. M. and Hemler, M. E. (1995). Integrin-ligand binding. Do integrins use a 'MIDAS touch' to grasp an Asp? Curr. Biol. 5, 615- 617.
6. Brooks, P. C., Stromblad, S., Sanders, L. C., von Schalscha, T. L., Aimes, R. T., Stetler-Stevenson, W. G., Quigley, J. P. and Cheresh, D. A. (1996). Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin alpha v beta 3. Cell 85, 683-693.
7. Byzova, T. V. and Plow, E. F. (1998). Activation of alphaVbeta3 on vascular cells controls recognition of prothrombin. J. Cell Biol. 143, 2081-2092.
8. Clark, K., Newham, P., Burrows, L., Askari, J. A. and Humphries, M. J. (2000). Production of recombinant soluble human integrin alpha4beta1. FEBS Lett. 471, 182-186.
9. Chen, Y. P., O'Toole, T. E., Shipley, T., Forsyth, J., LaFlamme, S. E., Yamada, K. M., Shattil, S. J. and Ginsberg, M. H. (1994). 'Inside-out' signal transduction inhibited by isolated integrin cytoplasmic domains. J. Biol. Chem. 269, 18307-18310.
10. Denda, S., Muller, U., Crossin, K. L., Erickson, H. P. and Reichardt, L. F. (1998). Utilization of a soluble integrin-alkaline phosphatase chimera to characterize integrin alpha 8 beta 1 receptor interactions with tenascin: murine alpha 8 beta 1 binds to the RGD site in tenascin-C fragments, but not to native tenascin-C. Biochemistry 37, 5464-5474.
11. D'Souza, S. E., Haas, T. A., Piotrowicz, R. S., Byers-Ward, V., McGrath, D. E., Soule, H. R., Cierniewski, C., Plow, E. F. and Smith, J. W. (1994). Ligand and cation binding are dual functions of a discrete segment of the integrin beta 3 subunit: cation displacement is involved in ligand binding. Cell 79, 659-667.
12. Diamond, M. S. and Springer, T. A. (1994). The dynamic regulation of integrin adhesiveness. Curr. Biol. 4, 506-517.
13. Du, X. P., Plow, E. F., Frelinger, A. L. d., O'Toole, T. E., Loftus, J. C. and Ginsberg, M. H. (1991). Ligands 'activate' integrin alpha lib beta 3 (platelet GPIIb-IIIa). Cell 65, 409-416.
14. Du, X., Saido, T. C., Tsubuki, S., Indig, F. E., Williams, M. J. and Ginsberg, M. H. (1995). Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit. J. Biol. Chem. 270, 26146-26151.
15. Dunon, D., Ruiz, P. and Imhof, B. A. (1993). Pro-T cell homing to the thymus. Curr. Topics Microbiol. Immunol. 184, 139-150.