Discrimination between NK and LAK cytotoxic activities of murine spleen cells by MTT assay: Differential inhibition by PGE2 and EDTA

Journal of Immunological Methods

Volumn 241, Issue 1-2, 2000, Pages 121-129

  Ribeiro Dias, Fátima ^a   Marzagão Barbuto, José Alexandre ^a   Tsujita, Maristela ^a   Jancar, Sonia ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Colorimetric assay; MTT; Murine NK LAK activity; PGE2

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; EDETIC ACID; PROSTAGLANDIN E2;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOCHEMISTRY; CYTOTOXICITY; IN VITRO STUDY; LYMPHOKINE ACTIVATED KILLER CELL; MALE; MOUSE; NATURAL KILLER CELL; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; TECHNIQUE;

ANIMALS; CYTOTOXICITY TESTS, IMMUNOLOGIC; CYTOTOXICITY, IMMUNOLOGIC; DINOPROSTONE; EDETIC ACID; KILLER CELLS, LYMPHOKINE-ACTIVATED; KILLER CELLS, NATURAL; MALE; MICE; MICE, INBRED C57BL; SPLEEN; TETRAZOLIUM SALTS; THIAZOLES;

MURINAE;

EID: 0034738652     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00206-4     Document Type: Article

References (28)

1. Arase H., Arase N., Saito T. Fas-mediated cytotoxicity by freshly isolated natural killer cells. J. Exp. Med. 181:1995;1235.
2. Berke G. Lymphocyte-triggered internal target disintegration. Immunol. Today. 12:1991;396.
3. Brunda M.J., Herberman R.B., Holden H.T. Inhibition of murine natural killer cell activity by prostaglandins. J. Immunol. 124:1980;2682.
4. 51Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 14:1968;181.
5. Callewaert D.M., Smeekens S.P., Mahle N.H. Improved quantification of cellular cytotoxicity reactions: determination of kinetic parameters for natural cytotoxicity by a distribution-free procedure. J. Immunol. Methods. 49:1982;25.
6. 2 and phosphodiesterase inhibitors on Con A blastogenic responses and NK cytotoxic activity of mouse spleen cells. J. Leukoc. Biol. 47:1990;371.
7. Ferrari M., Fornasiero M.C., Isetta A.M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J. Immunol. Methods. 131:1990;172.
8. Gardner C.M., Reen D.J. Differential cytokine regulation of natural killer cell-mediated necrotic and apoptotic cytotoxicity. Immunology. 93:1998;511.
9. Goldfarb R.H., Wasserman K., Herberman R.B., Kitson R.P. Nongranular proteolytic enzymes of rat IL-2-activated natural killer cells. J. Immunol. 149:1992;2061.
10. Henney C.S., Kuribayashi K., Kern D.E., Gillis S. Interleukin-2 augments natural killer cell activity. Nature. 291:1981;335.
11. Heo D.S., Park J-G., Hata K., Day R., Herberman R.B., Whiteside T.L. Evaluation of tetrazolium-based semiautomatic colorimetric assay for measurement of human antitumor cytotoxicity. Cancer Res. 50:1990;3681.
12. Hiserodt J.C., Britvan L.J., Targan S.R. Characterization of the cytolytic reaction mechanism of the human natural killer (NK) lymphocyte: resolution into binding, programming, and killer cell-independent steps. J. Immunol. 129:1982;1782.
13. Hussain R.F., Nouri A.M.E., Oliver R.T.D. A new approach for measurement of cytotoxicity using colorimetric assay. J. Immunol. Methods. 160:1993;89.
14. Lee R.K., Spielman J., Zhao D.Y., Olsen K.J., Podack E.R. Perforin, fas ligand, and tumor necrosis factor are the major cytotoxic molecules used by lymphokine-activated killer cells. J. Immunol. 157:1996;1919.
15. 2. Cell. Immunol. 123:1989;384.
16. Li H., Pohler U., Strehlow I., Hertig S., Baccarini M., Emmendörffer A., Tschopp J., Lohmann-Matthes M-L. Macrophage precursor cells produce perforin and perform YAC-1 lytic activity in response to stimulation with interleukin-2. J. Leukoc. Biol. 56:1994;117.
17. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:1983;55.
18. Nouri A.M.E., Mansouri M., Hussain R.F., Dos Santos A.V.L., Oliver R.T.D. Super-sensitive epithelial cell line and colorimetric assay to replace the conventional K562 target and chromium release assay for assessment of non-MHC-restricted cytotoxicity. J. Immunol. Methods. 180:1995;63.
19. Phillips J.H., Lanier L.L. Dissection of the lymphokine-activated killer phenomenon. Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis. J. Exp. Med. 164:1986;814.
20. Pross H.F., Maroun J.A. The standardization of NK cell assays for use in studies of biological response modifiers. J. Immunol. Methods. 68:1984;235.
21. Quan P.-C., Ishizaka T., Bloom B.R. Studies on the mechanism of NK cell lysis. J. Immunol. 128:1982;1786.
22. Tada H., Shiho O., Kuroshima K-I., Koyama M., Tsukamoto K. An improved colorimetric assay for interleukin 2. J. Immunol. Methods. 93:1986;157.
23. Trinchieri G. Biology of natural killer cells. Adv. Immunol. 47:1989;187.
24. 2 and forskolin. Cancer Immunol. Immunother. 40:1995;65.
25. van de Loosdrecht A.A., Nennie E., Ossenkoppele G.J., Beelen R.H.J., Langenhuijsen M.M.A.C. Cell mediated cytotoxicity against U937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study. J. Immunol. Methods. 141:1991;15.
26. Virág L., Kerékgyártó C., Fachet J. A simple, rapid and sensitive fluorimetric assay for the measurement of cell-mediated cytotoxicity. J. Immunol. Methods. 185:1995;199.
27. Yang J.C., Mulé J.J., Rosenberg S.A. Murine lymphokine-activated killer (LAK) cells: phenotype characterization of the precursor and effector cells. J. Immunol. 137:1986;715.
28. Zanovello P., Rosato A., Bronte V., Cerundolo V., Treves S., Di Virgilio F., Pozzan T., Biasi G., Collavo D. Interaction of lymphokine-activated killer cells with susceptible targets does not induce second messenger generation and cytolytic granule exocytosis. J. Exp. Med. 170:1989;665.