Cloning and expression of rat neutral sphingomyelinase: Enzymological characterization and identification of essential histidine residues

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1485, Issue 2-3, 2000, Pages 236-246

  Mizutani, Yukiko ^a   Tamiya Koizumi, Keiko ^a   Irie, Fumitoshi ^b   Hirabayashi, Yoshio ^b   Miwa, Masao ^c   Yoshida, Shonen ^a  

^a Res Inst Dis Mechanism Contr   (Japan)

^b Institute of Physical and Chemical Research   (Japan)

^c UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Active site; cDNA cloning; Lyso platelet activating factor; Neutral sphingomyelinase; Rat; Site directed mutagenesis

Indexed keywords

1 O ALKYLGLYCERO 3 PHOSPHORYLCHOLINE; COMPLEMENTARY DNA; DITHIOTHREITOL; HISTIDINE; MAGNESIUM; POTASSIUM CHLORIDE; SPHINGOMYELIN; SPHINGOMYELIN PHOSPHODIESTERASE; TYLOXAPOL;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; LIPID METABOLISM; MOLECULAR CLONING; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION; HISTIDINE; HUMANS; MALE; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLATELET ACTIVATING FACTOR; RATS; RATS, SPRAGUE-DAWLEY; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SPHINGOMYELIN PHOSPHODIESTERASE;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA; RODENTIA;

EID: 0034738089     PISSN: 13881981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1388-1981(00)00059-7     Document Type: Article

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