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Volumn 287, Issue 5462, 2000, Pages 2492-2493

Similar requirements of a plant symbiont and a mammalian pathogen for prolonged intracellular survival

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; GENE PRODUCT;

EID: 0034737819     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5462.2492     Document Type: Article
Times cited : (148)

References (25)
  • 10
    • 0342769853 scopus 로고    scopus 로고
    • note
    • The complete R. meliloti bacA ORF (GenBank X73522) was used to probe a B. abortus 2308 Lambda ZAP II library (Stratagene) and a 2308 genomic Southern blot.
  • 12
    • 0343204537 scopus 로고    scopus 로고
    • unpublished data
    • + reconstructed strains were selected by standard methods. Strain KL7R, the resulting restored wild-type strain, was used for further studies.
    • Phillips, R.W.1    LeVier, K.2
  • 13
    • 0029061955 scopus 로고
    • + reconstructed strains were selected by standard methods. Strain KL7R, the resulting restored wild-type strain, was used for further studies.
    • (1995) Gene , vol.158 , pp. 15
    • Schweizer, H.P.1    Hoang, T.T.2
  • 14
    • 0342769852 scopus 로고    scopus 로고
    • note
    • Saturated B. abortus cultures grown with antibiotics were adjusted to an optical density at 600 nm of 0.2, and a 100-μl sample was spread onto SA plates with antibiotics. Filter paper disks (10-mm diameter) soaked with 10 μ1 of 3 U/ml bleomycin sulfate (Sigma) in dimethylsulfoxide were placed on the plates and zones of growth inhibition measured.
  • 15
    • 0342769850 scopus 로고    scopus 로고
    • note
    • B. abortus strains opsonized with hyperimmune serum were added to resident peritoneal macrophages isolated from 8-to 10-week-old female BALB/c mice (Harlan Sprague Dawley) at an approximate ratio of 50 bacteria per macrophage in a gentimycin protection assay (21).
  • 16
    • 0342769851 scopus 로고    scopus 로고
    • note
    • 4 colony-forming units of B. abortus strains (21). At selected time points, five mice from each experimental group were killed and the spleens and left lateral lobes of the livers cultured. The total number of brucellae per organ was determined by serial dilution and plating on SBA.
  • 21
    • 0029035765 scopus 로고
    • For example, L. G. Rahme et al., Science 268, 1899 (1995).
    • (1995) Science , vol.268 , pp. 1899
    • Rahme, L.G.1
  • 24
    • 0343204535 scopus 로고    scopus 로고
    • unpublished data
    • V. K. Grippe, unpublished data.
    • Grippe, V.K.1
  • 25
    • 0342769849 scopus 로고    scopus 로고
    • note
    • We thank the lab of T. A. Ficht (Texas A&M University) for the B. abortus Lambda ZAP library. Supported by U.S. Public Health Service grant GM31030 from the National Institute of General Medical Science (G.C.W) and contracts from the U.S. Army Medical Research and Materiel Command (DAMD17-94-C-4054 and DAMD17-98-C-8045) (R.M.R. II, R.W.P., and V.K.G.). K.L. is a U.S. Department of Energy-Biosciences Fellow of the Life Sciences Research Foundation. The views, opinions, and/or findings contained in this report should not be construed as an official Department of the Army position, policy, or decision unless so designated by other documentation. Adherence to NIH Publication No. 86-23 (Revised 1985 USDHHS, 1985) was used for all experiments involving mice.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.