Western blotting for detection of glucocorticoid receptors in the brain and pituitary gland from adrenal intact pigs

Brain Research

Volumn 869, Issue 1-2, 2000, Pages 130-136

  Weaver, Shelley A ^a   Schaefer, Allan L ^b   Dixon, Walter T ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b LACOMBE RESEARCH CENTRE   (Canada)

Author keywords

Brain; Glucocorticoid receptor; Pig; Pituitary gland; Western blotting

Indexed keywords

11BETA,17BETA DIHYDROXY 6 METHYL 17ALPHA (1 PROPYNYL)ANDROSTA 1,4,6 TRIEN 3 ONE; CELL EXTRACT; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR;

ADRENAL GLAND; ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN REGION; CONTROLLED STUDY; ELECTROPHORESIS; HIPPOCAMPUS; HYPOPHYSIS; HYPOTHALAMUS; HYPOTHALAMUS HYPOPHYSIS ADRENAL SYSTEM; MALE; NONHUMAN; PREFRONTAL CORTEX; PRIORITY JOURNAL; SWINE; WESTERN BLOTTING;

ADRENAL GLANDS; ANIMALS; BLOTTING, WESTERN; BRAIN; MALE; PITUITARY GLAND; RECEPTORS, GLUCOCORTICOID; STRESS; SWINE;

EID: 0034733976     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(00)02358-1     Document Type: Article

References (38)

1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
2. Bresnick E.H., Dalman F.C., Sanchez E.R., Pratt W.B. Evidence that the 90-kDa heat shock protein is necessary for the steroid binding conformation of the L cell glucocorticoid receptor. J. Biol. Chem. 264:1989;4992-5007.
3. Burnstein K., Jewell C., Cidlowski J. Evaluation of the role of ligand and thermal activation on specific DNA binding by in vitro synthesized human glucocorticoid receptor. Mol. Endocrinol. 5:1991;1013-1022.
4. Olfert E.D., Cross B.M., McWilliams A.A. 2nd Edition A Guide To the Care and Use of Experimental Animals. Vol. 1:1993;Canadian Council on Animal Care, Ottawa.
5. Chen N.X., White B.D., Hausman G.J. Glucocorticoid receptor binding in porcine preadipocytes during development. J. Anim. Sci. 73:1995;722-727.
6. Cidlowski J., Bellingham D., Powell-Oliver F., Lubahn D., Sar M. Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors. Mol. Endocrinol. 4:1990;1427-1437.
7. De Kloet E.R. Brain corticosteroid receptor balance and homeostatic control. Front. Neuroendocrinol. 12:1991;95-164.
8. Diorio D., Viau V., Meaney M.J. The role of the medial prefrontal cortex (cingulate gyrus) in the regulation of hypothalamic-pituitary-adrenal responses to stress. J. Neurosci. 9:1993;3839-3847.
9. Dong Y., Poellinger L., Gustafsson J., Okrett S. Regulation of glucocorticoid receptor expression: evidence for transcriptional and posttranslational mechanisms. Mol. Endocrinol. 2:1988;1256-1264.
10. Hennessy D.P., Stelmasdiak T., Johnston N.E., Jackson P.N., Outch K.H.H. Consistent capacity for adrenocortical response to ACTH administration in pigs. Am. J. Vet. Res. 49:1988;1276-1283.
11. Hollenberg S., Weinberger C., Ong E., Cerelli G., Oro A., Lebo R., Thompson E., Rosenfeld M., Evans R. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature. 318:1985;635-641.
12. Issa A.M., Rowe W., Gauthier S., Meaney M.J. Hypothalamic-pituitary-adrenal activity in aged, cognitively impaired and cognitively unimpaired rats. J. Neurosci. 10:1990;3247-3254.
13. Kanitz E., Manteuffel G., Otten W. Effects of weaning and restraint stress on glucocorticoid receptor binding capacity in limbic areas of domestic pigs. Brain Res. 804:1998;311-315.
14. Keller-Wood M., Dallman M. Corticosteroid inhibition of ACTH secretion. Endocrinol. Rev. 5:1984;1-24.
15. Killian D.B., Kiesling D., Wulff F.P., Stewart A.N.V. Effects of adrenalectomy and glucocorticoids on puberty in gilts reared in confinement. J. Anim. Sci. 64:1987;231-236.
16. Klemcke H.G. Responses of the porcine pituitary-adrenal axis to chronic intermittent stressor. Dom. Anim. Endocrinol. 11:1994;133-149.
17. Klemcke H.G., Nienaber J.A., Hahn G.L. Plasma adrenocorticotropic hormone and cortisol in pigs: effects of time of day on basal and stressor-altered concentrations. Proceedings of the Society for Experimental Biology and Medicine. 190:1989;42-53.
18. 14C in polyacrylamide gels by fluorography. Eur. J. Biochem. 56:1975;334-341.
19. Marks A.R., Moore D.D., Buckley D.I., Gametchu B., Goodman H.M. Conservation of the DNA binding domain and other properties between porcine and rat glucocorticoid receptors. J. Steroid Biochem. 6:1986;1097-1103.
20. McEwen B.S., Wallach G., Magnus C. Corticosterone binding to hippocampus: immediate and delayed influences of the absence of adrenal secretion. Brain Res. 70:1974;321-334.
21. 3H]Dexamethasone binding in rat frontal cortex. Brain Res. 328:1985;176-180.
22. Meaney M.J., Aitken D.H., Bodnoff S.R., Iny L.J., Sapolsky R.M. The effects of postnatal handling on the development of the glucocorticoid receptor systems and stress recovery in the rat. Neuro-Psychopharmacol. Biol. Psychiat. 9:1985;731-734.
23. Meaney M.J., Viau V., Aitken D.H., Bhatnagar S. Stress-induced occupancy and translocation of hippocampal glucocorticoid receptors. Brain Res. 445:1988;198-203.
24. Meaney M.J., Viau V., Aitken D.H., Bhatnagar S. Glucocorticoid receptors in brain and pituitary of the lactating rat. Physiol. Behav. 45:1989;209-212.
25. Meaney M.J., O'Donnell D., Viau V., Bhatnagar S., Sarrieau A., Smythe J., Shanks N., Walker C. Corticosteroid receptors in the rat brain and pituitary during development and hypothalamic-pituitary-adrenal function. Zagon I.S., McLaughlin P.J. Receptors in the Developing Nervous System. Vol. 1: Growth Factors and Hormones:1993;163-201 Chapman and Hall, London.
26. Oakley R.H., Sars M., Cidlowski J.A. The human glucocorticoid receptor β isoform. J. Biol. Chem. 271:1996;9550-9559.
27. O'Donnell D., Francis D., Weaver S., Meaney M.J. Effects of adrenalectomy and corticosterone replacement on glucocorticoid receptor levels in rat brain tissue: a comparison between Western blotting and receptor binding assays. Brain Res. 687:1995;133-142.
28. Pratt W.B. The role of heat shock proteins in regulating the function, folding, and trafficking of the glucocorticoid receptor. J. Biol. Chem. 268:1993;21455-21458.
29. r 90 000 heat shock protein: an evolving model of ligand-mediated receptor transformation and translocation. Cancer Res. 49:1989;2222s-2229s.
30. Sapolsky R.M., Krey L.C., McEwen B.S. Glucocorticoid-sensitive hippocampal neurons are involved in terminating the adrenocortical stress response. Proc. Natl. Acad. Sci. USA. 81:1984;6174-6177.
31. Sapolsky R.M., Meaney M.J., McEwen B.S. The development of the glucocorticoid receptor system in the rat limbic brain. III. Negative-feedback regulation. Dev. Brain Res. 18:1985;169-173.
32. Scherrer L.C., Dalman F.C., Massa E., Meshinchi S., Pratt W.B. Structural and functional reconstitution of the glucocorticoid receptor-Hsp90 complex. J. Biol. Chem. 265:1990;21397-21400.
33. Spencer R.L., Young E.A., Choo P.H., McEwen B.S. Adrenal steroid type I and type II receptor binding: estimates of in vivo receptor number, occupancy, and activation with varying level of steroid. Brain Res. 514:1990;37-48.
34. Spencer R.L., Miller A.H., Moday H., Stein M., McEwen B.S. Diurnal differences in basal and acute stress levels of type I and type II adrenal steroid receptor activation in neural and immune tissues. Endocrinology. 133:1993;1941-1950.
35. Srivastava D., Thompson E.B. Two glucocorticoid binding sites on the human glucocorticoid receptor. Endocrinology. 127:1990;1770-1778.
36. Towbin H., Staehelin T., Gordon T. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
37. Weatherill P.J., Bell P.A. Comparison of the physical characteristics of the molybdate-stabilized glucocorticoid receptor from rat, pig and human tissues. J. Steroid Biochem. 21:1984;287-291.
38. Yarney T.A., Kendall J.Z., Randall G.C.B. Cytosolic glucocorticoid receptors in the porcine lung during development and after hypophysectomy or thyroidectomy. J. Endocrinol. 127:1990;341-349.