Hydrophobic clustering in acid-denatured IL-2 and fluorescence of a Trp NH···π H-bond

Biochemical and Biophysical Research Communications

Volumn 279, Issue 3, 2000, Pages 770-778

  Nanda, Vikas ^a   Liang, Shu Mei ^b   Brand, Ludwig ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

Author keywords

Dielectric relaxation; Heterogeneity; Molten globule; Time resolved fluorescence; Tryptophan

Indexed keywords

INTERLEUKIN 2; TRYPTOPHAN;

ARTICLE; CHEMICAL BINDING; ELECTRICITY; FLUORESCENCE SPECTROSCOPY; HUMAN; HYDROGEN BOND; HYDROPHOBICITY; PH; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN INTERACTION; QUANTUM MECHANICS; WAVEFORM;

EID: 0034731308     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.4033     Document Type: Article

References (50)

1. Evidence for identity between the equilibrium unfolding intermediate and a transient folding intermediate: A comparative study of the folding reactions of α-lactalbumin and lysozyme
2. Formation of a molten globule intermediate early in the kinetic folding pathway of apomyoglobin
3. Protein folding: Hypotheses and experiments
4. Protein folding and protein evolution: Common folding nucleus in different subfamilies of c-type cytochromes?
5. 19F-NMR study
6. Hydrophobic clustering in nonnative states of a protein: Interpretation of chemical shifts in NMR spectra of denatured states of lysozyme
7. Formation of a hydrophobic cluster in denatured bovine pancreatic trypsin inhibitor
8. Time-resolved fluorescence studies of the molten globule state of apomyoglobin
9. Pressure-induced perturbation of apomyoglobin structure: Fluorescence studies on native and acidic compact forms
10. Nanosecond dynamics of tryptophans in different conformational states of apomyoglobin proteins
11. Inter- and intramolecular interactions of α-lactalbumin. III. Spectral changes at acid pH
12. The IL-2 receptor complex: Its structure, function and target genes
13. Unraveling the structure of IL-2
14. Response to: Unraveling the structure of IL-2
15. The protein data bank: A computer-based archival file for macromolecular structure
16. Aromatic interactions in homeodomains contribute to the low quantum yield of a conserved, buried tryptophan
17. Picosecond study of solute-solvent interaction of excited-state of indole
18. Immunosupressor binding to the immunophilin FKBP59 affects the local structure dynamics of a surface β-strand: Time-resolved fluorescence study
19. Structure-activity relationships of recombinant human interleukin-2
20. Evidence for an acid-induced molten-globule state in interleukin-2; a fluorescence and circular dichroism study
21. Interaction of recombinant interleukin-2 with liposomal bilayers
22. Studies of structure-activity relationships of human interleukin-2
23. Folding and purification of insoluble (inclusion-body) proteins from Escherichia coli
24. Characterization of recombinant human interleukin-2 derived from Escherichia coli
25. How to measure and predict the molar absorption coefficient of a protein
26. On the analysis of fluorescence decay kinetics by the method of least-squares
27. 2+ into liposomes
28. Study of the conformation in the excited-state of two tryptophanyl diketopiperazines
29. Time-resolved fluorescence of the two tryptophans in horse liver alcohol dehydrogenase
30. Decay-associated fluorescence spectra and the heterogeneous emission of alcohol dehydrogenase
31. Nanosecond time-resolved fluorescence spectra of a protein-dye complex
32. Supersonic beam studies of hydrogen-bonded indoles: Relative interaction strengths
33. Two-color threshold photoionization spectroscopy of jet-cooled indole clusters
34. Van Der Waals versus hydrogen-bonding in complexes of indole with argon, water, and benzene by mass-analyzed pulsed field threshold ionization
35. Solvent effects in the fluorescence of indole and substituted indoles
36. Hydrogen bonding and charge transfer interactions in exciplexes formed by excited indole and monosubstituted benzenes in cyclohexane
37. Three-dimensional structure of the immunophilin-like domain of FKBP59 in solution
38. Tryptophan dynamics of the FK506 binding protein: Time-resolved fluorescence and simulations
39. Equilibrium denaturation of recombinant human FK binding protein in urea
40. Tryptophan microstate reshuffling upon the binding of cyclosporin A to human cyclophilin A
41. Secondary structure of human interleukin-2 from 3D heteronuclear NMR experiments
42. Influence of conformation on the fluorescence of tryptophan-containing peptides
43. 2]oxytocin
44. Time-resolved nanosecond emission spectroscopy: Spectral shifts due to solvent-solute relaxation
45. Nanosecond time-resolved emission spectroscopy of a fluorescence probe adsorbed to L-α-egg lecithin vesicles
46. Dipolar relaxation in proteins on the nanosecond timescale observed by wavelength-resolved phase fluorometry of tryptophan fluorescence
47. Solvent relaxation around the excited state of indole: Analysis of fluorescence lifetime distributions and time-dependent spectral shifts
48. Motionally restricted tryptophan environments at the peptide-lipid interface of gramicidin channels
49. Spectrally- and time-resolved fluorescence emission of indole during solvent relaxation: A quantitative model