Molecular mechanisms for antibody-mediated modulation of peptide-displaying enzyme sensors

Biochemical and Biophysical Research Communications

Volumn 275, Issue 2, 2000, Pages 360-364

  Ferrer Miralles, Neus ^a   Feliu, Jordi X ^a   Villaverde, Antonio ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Biosensor; Enzymatic modulation; Insertional mutants; k(cat)

Indexed keywords

ALKALINE PHOSPHATASE; ANTIBODY; ANTIGEN; BETA GALACTOSIDASE; BETA LACTAMASE;

ARTICLE; BIOSENSOR; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ESCHERICHIA COLI; GENETIC ENGINEERING; LIGAND BINDING; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; STEREOCHEMISTRY;

EID: 0034726728     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3309     Document Type: Article

References (16)

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3. A molecular sensor system based on genetically engineered alkaline phosphatase
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5. Distinct mechanisms of antibody-mediated enzymatic reactivation in beta-galactosidase molecular sensors
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7. Corrigendum to: Distinct mechanisms of antibody-mediated enzymatic reactivation in β-galactosidase molecular sensors
8. Insertion of a 27 amino acid viral peptide in different zones of Escherichia coli beta-galactosidase: Effects on the enzyme activity
9. Engineering of solvent-exposed loops in Escherichia coli beta-galactosidase
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11. One-step purification of hybrid proteins which have β-galactosidase activity
12. Uses of beta-galactosidase tag in online monitoring production of fusion proteins and gene expression in Escherichia coli
13. Characterization of random-sequence proteins displayed on the surface of Escherichia coli RNase HI
14. Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution
15. Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein
16. Structure and function of the Bacillus hybrid enzyme GluXyn-1: Native-like jellyroll fold preserved after insertion of autonomous globular domain