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Volumn 289, Issue 5479, 2000, Pages 617-619

Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN KINASE; RECEPTOR; TRANSCRIPTION FACTOR;

EID: 0034725925     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5479.617     Document Type: Article
Times cited : (918)

References (26)
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    • T. A. S. Steeves and I. M. Sussex, Patterns in Plant Development (Cambridge Univ. Press, New York, 1989); S. E. Clark, Semin. Cell Dev. Biol. 7, 873 (1996); E. M. Meyerowitz, Cell 88, 299 (1997); P. Laufs, O. Grandjean, C. Jonak, K. Kieu, J. Traas, Plant Cell 10, 1375 (1998).
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    • T. A. S. Steeves and I. M. Sussex, Patterns in Plant Development (Cambridge Univ. Press, New York, 1989); S. E. Clark, Semin. Cell Dev. Biol. 7, 873 (1996); E. M. Meyerowitz, Cell 88, 299 (1997); P. Laufs, O. Grandjean, C. Jonak, K. Kieu, J. Traas, Plant Cell 10, 1375 (1998).
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  • 15
    • 0027435506 scopus 로고
    • A CLV3 full-length cDNA was inserted between the CaMV35S promoter and the 3′NOS terminator of pRTΩNot I to give pBU4. Clone orientation and fidelity was verified by sequencing. A Pst I restriction fragment of pBU4 was introduced into pGREEN (P. Mullineaux and R. Hellens, John Innes Centre, Norwich, UK) to give pBU6 (CaMV35S::CLV3). pBU6 was transformed into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis plants by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C. R. Acad. Sci. Paris Sci. Vie 316, 1194 (1993)]. Transformation efficiency ranged from 0.5 to 5.0%.
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    • note
    • Some 2% of all transgenic plants presumably failed to express the transgene at sufficient levels to give a phenotype.
  • 17
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    • note
    • In situ hybridizations were performed as described in (2).
  • 18
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    • note
    • A 5.5-kb Eco RI genomic DNA fragment containing the CLV3 gene was cloned into pGREEN, giving gE55CLV3, and transformed into Agrobacterium strain GV3101. Introduction of gE55CLV3 into clv3-2 mutants rescued the mutant phenotype. Plants that carried four copies of the CLV3 gene were obtained by introducing two copies of gE55CLV3 into the genome of wild-type plants.
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  • 21
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    • note
    • A DNA fragment containing 3.5-kb DNA upstream of the putative transcription start site of the UFO gene was used to replace the CaMV35S promoter in pRTΩNot I. A CLV3 cDNA clone was then inserted between the Ω-leader and the NOS-terminator giving pBU11. The UFO::CLV3 gene construct was transferred as a Pst I fragment into a pGREEN T-DNA vector, giving pBU12. Transformation of Arabidopsis wild type and clv3-8 mutants via Agrobacterium GV3101 was performed as described above. We analyzed 203 transformants.
  • 23
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    • Schoof, H.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.