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0343842777
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The IG16 and IC17 lines of heterozygous transgenic mice and their wild-type littermates (22) were deeply anesthetized with sodium pentobarbital at the ages of 7 to 13 weeks. A glass needle was introduced into the left hemisphere with stereotaxic techniques (24), and the anti-Tac(Fv)-PE38 immunotoxin (10 ng/0.5 μl of PBS) or PBS alone was slowly injected into five points in the left striatum over 3 min. For 6-OHDA treatment, PBS (0.5 μl) containing 6-OHDA hydrobromide (4 mg/ml) and 0.016% ascorbic acid was injected into two points in the left striatum over 1 min. All procedures were performed according to guidelines of Kyoto University Faculty of Medicine.
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unpublished data
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ACh contents in the striatum were measured with radioimmunoassay as described by K. Kawashima, H. Ishikawa, and M. Mochizuki [J. Pharmacol. Methods 3, 115 (1980)]. DA, DOPAC, and HVA contents in the striatum were measured with high-performance liquid chromatography as described by M. Warnhoff [J. Chromatogr. 307, 271 (1984)]. Levels of ACh, DA, DOPAC, and HVA in the intact striatum of wild-type mice were 760 ± 50, 616 ± 24, 29.0 ± 0.9, and 31.7 ± 1.3 pmol/mg of protein (mean ± SEM), respectively. Statistical analysis was performed by analysis of variance, and post hoc comparisons for this analysis and for histological and behavioral analysis were made with Fisher's protected least significant difference and Scheffé test, respectively.
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0021255346
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ACh contents in the striatum were measured with radioimmunoassay as described by K. Kawashima, H. Ishikawa, and M. Mochizuki [J. Pharmacol. Methods 3, 115 (1980)]. DA, DOPAC, and HVA contents in the striatum were measured with high-performance liquid chromatography as described by M. Warnhoff [J. Chromatogr. 307, 271 (1984)]. Levels of ACh, DA, DOPAC, and HVA in the intact striatum of wild-type mice were 760 ± 50, 616 ± 24, 29.0 ± 0.9, and 31.7 ± 1.3 pmol/mg of protein (mean ± SEM), respectively. Statistical analysis was performed by analysis of variance, and post hoc comparisons for this analysis and for histological and behavioral analysis were made with Fisher's protected least significant difference and Scheffé test, respectively.
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0343406822
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note
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Mice were placed in a round-bottom glass bowl (25 cm in diameter), and rotations were counted for a 5-min period by visual observation. One rotation was defined by the animal completing a 360° circle without turning back to the opposite direction. Apomorphine-HCl (1 mg/kg) and haloperidol (6 mg/kg) were injected 15 min before measuring rotations.
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0342971569
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note
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35S]-riboprobe and digoxigenin-labeling hybridization was performed according to procedures described previously (23) and those described by Le Moine and Bloch (37), respectively.
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34
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0342537313
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note
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3H]-ligand binding was performed as described by Berger et al. (17) with a minor modification. Quantification of autoradiographs was done essentially as described for in situ hybridization analysis.
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39
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0342971563
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note
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3H]-ACh. This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan and the International Resource Program of the National Cancer Institute.
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