DNA annealing and DNA-protein interactions by capillary electrophoresis

Biochemical and Biophysical Research Communications

Volumn 272, Issue 1, 2000, Pages 251-258

  Singhal, Ram P ^b   Otim, Ochan ^a,b  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b WICHITA STATE UNIVERSITY   (United States)

Author keywords

Association constants; Binding constant; Binding constants; Capillary electrophoresis; DNA conformation changes measured by CE; DNA protein binding; DNA protein interactions; MEKC; Mobility shift gel electrophoresis assay; On column annealing; On column DNA protein complex formation

Indexed keywords

FLUORESCEIN; SINGLE STRANDED DNA;

ARTICLE; BINDING SITE; CAPILLARY ELECTROPHORESIS; DISSOCIATION CONSTANT; DNA PROBE; DNA PROTEIN COMPLEX; GEL ELECTROPHORESIS; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

EID: 0034720441     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.2720     Document Type: Article

References (23)

1. Taylor, A., Erba, H. P., Muscat, G. E. O., and Kedes, L. (1988) Genomics 3, 323-336.
2. Singhal, R. P., and Xian, J. (1993) J. Chromatogr. A 562, 47-56.
3. Otim, O., and Singhal, R. P. (1997) J. Cap. 4, 247-251.
4. Grossman P. D. (1994) J. Chromatogr. A 663, 219-227.
5. Winzor, D. J. (1995) J. Chromatogr. A 696, 160-163.
6. Rundlett, K. L., and Armstrong, D. W.. (1996) J. Chromatogr. A 721, 173-786.
7. Szoko, E., Gyimesi, J., Barcza, L., and Magyar, K. (1996) J. Chromatogr. A 745, 181-187.
8. Busch, M. H. A., Boelens, H. F. M., Kraak, J. C., and Poppe, H. (1997) J. Chromatogr. A 775, 313-326.
9. Busch, M. H. A., Kraak, J. C., and Poppe, H. (1997) J. Chromatogr. A 777, 329-353.
10. Hancock, R. (1997) Analyst 122, 51R-58R.
11. Foulds, G. J., and Etzkorn, F. A. (1999) J. Chromatogr. A 862, 231-236.
12. Hamdan, I. I., Skellern, G. G., and Waigh, R. D. (1998) Nucleic Acids Res. 26, 3053-3058.
13. Xue, B., Gabrielsen, and Myrset, A. H. (1997) J. Cap. Elec. 4, 225-231.
14. Li, C., and Martin, L. M. (1998) Anal. Biochem. 263, 72-78.
15. Foulds, G. J., and Etzkorn, F. A. (1998) Nucleic Acids Res. 26, 4304-4305.
16. Cann, J. R. (1998) Electrophoresis 19, 1336-1340.
17. Fried, M. G., and Daugherty, M. A. (1998) Electrophoresis 19, 1247-1253.
18. Xian, J. (1994) Application of Capillary Electrophoresis for the Separation of Biomolecules, Ph.D. dissertation, pp. 81-83, Wichita State University, Wichita, Kansas.
19. Singhal, R. P., Xian, J., and Otim, O. (1996) J. Chromatogr. A 756, 263-277.
20. Calzone, F. J., Hg, C. H., Teplow, D. B., Cutting, A. E., Zeller, R. W., Britten, R. J., and Davidson, E. H., (1991) Development 112, 335-350.
21. Hoog, C., Calzone, F. J., Cutting, A. E., Britten, R. J., and Davidson, E. H. (1991) Development 112, 351-364.
22. Xian, J., Harrington, M. G., and Davidson, E. H. (1996) Proc. Natl. Acad. Sci. USA 93, 86-90.
23. Davidson, E. H. (1986) Gene Activity in Early Development, 3rd ed., Academic Press, Orlando, FL.