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Volumn 122, Issue 29, 2000, Pages 6848-6854

Structural basis for reaction mechanism and drug delivery system of chromoprotein antitumor antibiotic C-1027

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC C 1027; ANTINEOPLASTIC AGENT; APOPROTEIN; CARRIER PROTEIN;

EID: 0034718061     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja994323a     Document Type: Article
Times cited : (26)

References (32)
  • 23
    • 0343101273 scopus 로고    scopus 로고
    • note
    • We used the structure coordinates of the Chr-Apo complex supplied by Dr. Ishiguro. The coodinates were derived for the aromatized Chr-Apo complex.
  • 24
    • 0028557126 scopus 로고
    • Matsumoto, T.; Sugiura, Y. Biochem. Biophys. Res. Commun. 1994, 205, 1533-1538. In preliminary experiments of DNA-Chr titration, we tried various kinds of DNA oligomers containing GTTAT/ATAAC, CTTTT/ AAAAG, ATAAT/ATTAT, CTTTA/TAAAG, and CTCJT/AAGAG. However, the imino proton-spectral region of complexes of Chrs with most oligomers except for d(TGCCATC)/d(GATGGCA) exhibited nonstoichiometric or broadening resonaces, indicating formation of multiple conformations of complex in each case. Namely, other duplexes except for d(TGCCATC)/d(GATGGCA) contain multiple binding sites despite single cleavage site by the Chr. The drug bound to a DNA sequence cannot always abstract the hydrogen atoms of the DNA nucleotides. Therefore, the 2-amino group of the central G is important to enhance specificity of DNA target recognition (not cleavage) by C1027-Chr.
    • (1994) Biochem. Biophys. Res. Commun. , vol.205 , pp. 1533-1538
    • Matsumoto, T.1    Sugiura, Y.2
  • 25
    • 0343101271 scopus 로고    scopus 로고
    • note
    • lida and Hirama reported that the constraint of the nine-membered ring of C1027-Chr may contribute to lowering the barrier for interconversion between the enediyne and biradical forms, and consequently the reaction process becomes reversible at ambient temperature. The hydrogen abstraction step by a biradical form is kinetically significant in the cycloaromatization of C1027-Chr. Therefore, the reactivity of C-1027 is affected more directly by conformation of aromatized form analogous to the biradical intermediate rather than by that of enediyne form.
  • 29
    • 0342666963 scopus 로고    scopus 로고
    • note
    • We used the benzene ring as a group adjcent to p-benzyne biradical for the purpose of the simplicity of ab initio calculation. If chlorophenol ring is employed instead of benzen ring, the functional substituent groups (chloro- and hydroxyl-group) may influence the magnitude of S-T splitting difference for the two C1027-Chr form. Therefore, the calculated S-T gap difference (~0.04) can indicate qualitative tendency of the C1027reactivity. More accurate calculations will lead to these quantitative interpretations.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.