An RT-PCR-based method for the diagnosis of the sleeping disease virus in experimentally and naturally infected salmonids

Diseases of Aquatic Organisms

Volumn 40, Issue 1, 2000, Pages 19-27

  Villoing, Stéphane ^a,b   Castric, Jeannette ^b   Jeffrey, Joëlle ^b   Le Ven, Alain ^b   Thiery, Richard ^b   Bremont, Michel ^a  

^a INRA Institut National de la Recherche Agronomique   (France)

^b Healthy Pigs Production and Experimentation Section   (France)

Author keywords

Alphavirus; Diagnosis; Rainbow trout; RT PCR; Salmon; Salmon pancreas disease virus; Sleeping disease virus

Indexed keywords

ALPHA VIRUS; DISEASE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMON PANCREAS DISEASE VIRUS; SLEEPING DISEASE VIRUS; VIRUS DETECTION; VIRUS INFECTION; VIRUS RNA;

ALPHAVIRUS; MYKISS; ONCORHYNCHUS MYKISS; PISCES; SALMON PANCREAS DISEASE VIRUS; SALMONIDAE; SLEEPING DISEASE VIRUS;

EID: 0034708203     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao040019     Document Type: Article

References (24)

1. Blake SL, Schiil WB, McAllister PE, Lee MK, Singer JT, Nicholson BL (1995) Detection and identification of aquatic birnaviruses by PCR assay. J Clin Microbiol 33:835-839
2. Boucher P, Baudin-Laurencin F (1994) Sleeping disease of salmonids. Bull Eur Assoc Fish Pathol 14:179-180
3. Boucher P, Baudin-Laurencin F (1996) Sleeping disease and pancreas disease comparative histopathology and acquired cross-protection. J Fish Dis19:303-310
4. Boucher P, Castric J, Baudin-Laurencin F (1994) Observation of virus-like particles in rainbow-trout Oncorhynchus mykiss infected with sleeping disease virulent material. Bull Eur Assoc Fish Pathol 14:215-216
5. Boucher P, Le Ven A, Baudin-Laurencin F (1995) Transmission expérimentale de la maladie du sommeil: histologie et caractérisation de l'agent infectieux. Nouv Sci Technol 13: 115-117
6. Bruchhof B, Marquardt O, Enzmann PJ (1995) Differential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction. J Virol Methods 55:111-119
7. Castric J, Baudin-Laurencin F, Brémont M, Jeffroy J, Le Ven A, Béarzotti M (1997) Isolation of the virus responsible for sleeping-disease in experimentally infected rainbow-trout (Oncorhynchus mykiss). Bull Eur Assoc Fish Pathol 17: 27-30
8. Gabe M (1968) Techniques histologiques. Masson, Paris
9. Guillou JP, Merle G, Henault S, Hattenberger AM (1999) Detection of viral hemorrhagic septicemia virus (VHSV) in rainbow trout (Oncorhynchus mykiss) by reverse transcription followed by polymerase chain reaction (Diagnostic validation). Vet Res 30:49-60
10. Griffin DE (1998) A review of alphavirus replication in neurons. Neurosci Biobehav Rev 22:721-723
11. Houghton G (1995) Acquired protection in Atlantic Salmon Salmo salar and post-smolts against pancreas disease. Dis Aquat Org 18:109-118
12. Lannan CN, Winton JR, Fryer JL (1984) Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20:671-676
13. Lopez-Lastra M, Gonzalez M, Jashes M, Sandino AM (1994) A detection method for infectious pancreatic necrosis virus (IPNV) based on reverse transcription (RT)-polymerase chain reaction (PCR). J Fish Dis 17:269-282
14. McLoughlin M (1997) The differential diagnosis of the major pancreatic disorders of salmonids, a diagnostic challenge. Bull Eur Assoc Fish Pathol 17:205
15. Miller TA, Rapp J, Wastlhuber U, Hoffmann RW, Enzmann PJ (1998) Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured cells. Dis Aquat Org 34:13-20
16. Murphy TM, Rodger HD, Drinan F, Cannon F, Kruse P, Korting W (1992) The sequential pathology of pancreas disease in atlantic salmon farms in Ireland. J Fish Dis 15: 401-408
17. Nelson RT, McLoughlin MF, Rowley HM, Platten MA, McCormick JI (1995) Isolation of a toga-like virus from farmed Atlantic salmon Salmo salar with pancreas disease. Dis Aquat Org 22:25-32
18. Nishiziwa T, Mori KI, Nakai T, Furusawa I, Muroga K (1994) Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Dis Aquat Org 18:103-107
19. Péducasse S, Castric J, Thiery R, Jeffroy J, Le Ven A, Baudin-Laurencin F (1999) Comparative study of viral encephalopathy and retinopathy in juvenile sea bass Dicentrarchus labrax infected in different ways. Dis Aquat Org 36:11-20
20. Strauss JH, Strauss EG (1994) The alphavirus: gene expression, replication, and evolution. Microbiol Rev 58:491-562
21. Suzuki S, Hosono N, Kusuda R (1997) Detection of aquatic birnavirus gene from marine fish using a combination of reverse transcription- and nested PCR. J Mar Biotechnol 5:205-209
22. Villoing S, Béarzotti M, Chilmonczyk S, Castric J, Brémont M (2000) Rainbow trout sleeping disease virus is an atypical alphavirus. J Virol 74:173-183
23. Weston JH, Welsh MD, McLoughlin MF, Todd D (1999) Salmon pancreas disease virus, an alphavirus infecting farmed atlantic salmon, Salmo salar L. Virology 256: 188-195
24. Wirz B, Tratschin JD, Muller HK, Mitchell DB (1993) Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction. J Clin Microbiol 31:1148-1154