nSec1 binds a closed conformation of syntaxin1A

Journal of Cell Biology

Volumn 148, Issue 2, 2000, Pages 247-252

  Yang, Bin ^a   Steegmaier, Martin ^a   Gonzalez Jr , Lino C ^a   Scheller, Richard H ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Exocytosis; Membrane fusion; SNARE; Synapse; Synaptic vesicles

Indexed keywords

NEUROTOXIN; SNARE PROTEIN; SYNAPTOBREVIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN;

ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; EXOCYTOSIS; GEL ELECTROPHORESIS; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; RAT; SYNAPSE VESICLE;

ANIMALS; ANTIGENS, SURFACE; BIOLOGICAL TRANSPORT; BOTULINUM TOXINS; MEMBRANE FUSION; MEMBRANE PROTEINS; MOLECULAR CHAPERONES; MUNC18 PROTEINS; NERVE TISSUE PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; R-SNARE PROTEINS; RATS; RECOMBINANT PROTEINS; SNARE PROTEINS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; SYNTAXIN 1; VESICULAR TRANSPORT PROTEINS;

ANIMALIA;

EID: 0034707644     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.148.2.247     Document Type: Article

References (27)

1. Bennett, M.K. 1995. SNAREs and the specificity of transport vesicle targeting. Curr. Opin. Cell Biol. 7:581-586.
2. Bennett, M.K., and R.H. Scheller. 1994. A molecular description of synaptic vesicle membrane trafficking. Annu. Rev. Biochem. 63:63-100.
3. Brennwald, P., B. Kearns, K. Champion, S. Keranen, V. Bankaitis, and P. Novick. 1994. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 79:245-258.
4. Calakos, N., and R.H. Serieller. 1994. Vesicle-associated membrane protein and synaptophysin are associated or the synaptic vesicle. J. Biol. Chem. 269: 24534-24537.
5. Calakos, N., M.K. Bennett, K.E. Peterson, and R.H. Scheller. 1994. Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking. Science. 263:1146-1149.
6. Carr, C.M., E. Grote, M. Munson, F.M. Hughson, and P.J. Novick. 1999. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J. Cell Biol. 146:333-344.
7. 2+ and drives membrane fusion. Cell. 97:165-174.
8. Cowles, C.R., S.D. Emr, and B.F. Horazdovsky. 1994. Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. J. Cell Sci. 107:3449-3459.
9. Dulubova, I., S. Sugita, S. Hill, M. Hosaka, I. Fernandez, T.C. Sudhof, and J. Rizo. 1999. A conformational switch in syntaxin during exocytosis: role of munc18. EMBO (Eur. Mol. Biol. Organ.) J. 18:4372-4382.
10. Fernandez, I., J. Ubach, I. Dulubova, X. Zhang, T.C. Sudhof, and J. Rizo. 1998. Three-dimensional structure of an evolutionary conserved N-terminal domain of syntaxin 1A. Cell. 94:841-849.
11. Garcia, E.P., E. Gatti, M. Butler, J. Burton, and P. De Camilli. 1994. A rat brain Sec1 homologue related to Rop and UNC18 interacts with syntaxin. Proc. Natl. Acad. Sci. USA. 91:2003-2007.
12. Hata, Y., C.A. Slaughter, and T.C. Sudhof. 1993. Synaptic vesicle fusion complex contains unc-18 homologue hound to syntaxin. Nature. 366:347-351.
13. Hayashi, T., H. McMahon, S. Yamasaki, T. Binz, Y. Hata, T.C. Sudhof, and H. Niemann. 1994. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. EMBO (Eur. Mol. Biol. Organ.) J. 13: 5051-5061.
14. Hodel, A., T. Schafer, D. Gerosa, and M.M. Burger. 1994. In chromaffin cells, the mammalian Sec1p homologue is a syntaxin 1A-binding protein associated with chromaffin granules. J. Biol Chem. 269:8623-8626.
15. Jahn, R., and T.C. Südhof. 1994. Synaptic vesicles and exocytosis. Annu. Rev. Neurosci. 17:219-246.
16. Novick, P., and R. Scheckman. 1979. Secretion and cell surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 76:1858-1862.
17. Ossig, R., C. Dascher, H.H. Trepte, H.D. Schmitt, and D. Gallwitz. 1991. The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. Mol. Cell. Biol. 11:2980-2993.
18. Peterson, M.R., C.G. Burd, and S.D. Emr. 1999. Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Curr. Biol. 9:159-162.
19. Pevsner, J., S.C. Hsu, J.E. Braun, N. Calakos, A.E. Ting, M.K. Bennett, and R.H. Scheller. 1994a. Specificity and regulation of a synaptic vesicle docking complex. Neuron. 13:353-361.
20. Pevsner, J., S.C. Hsu, and R.H. Scheller. 1994b. n-Sec1: a neural-specific syntaxin-binding protein. Proc. Natl. Acad. Sci. USA. 91:1445-1449.
21. Poirier, M.A., W. Xiao, J.C. Macosko, C. Chan, Y.K. Shin, and M.K. Bennett. 1998. The synaptic SNARE complex is a parallel four-stranded helical bundle. Nat. Struct. Biol. 5:765-769.
22. Rothman, J.E. 1994. Mechanisms of intracellular protein transport. Nature. 372: 55-63.
23. Schulze, K.L., J.T. Littleton, A. Salzberg, N. Halachmi, M. Stern, Z. Lev, and H.J. Bellen. 1994. rop, a Drosophila homolog of yeast Sec1 and vertebrate n-Sec1/Munc-18 proteins, is a negative regulator of neurotransmitter release in vivo. Neuron. 13:1099-1108.
24. Sutton, R.B., D. Fasshauer, R. Jahn, and A.T. Brunger. 1998. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution. Nature. 395:347-353.
25. Weber, T., B.V. Zemelman, J.A. McNew, B. Westermann, M. Gmachl, F. Parlati, T.H. Sollner, and J.E. Rothman. 1998. SNAREpins: minimal machinery for membrane fusion. Cell. 92:759-772.
26. Wu, M.N., J.T. Littleton, M.A. Bhal, A. Prokop, and H.J. Bellen. 1998. ROP, the Drosophila Sec1 homolog, interacts with syntaxin and regulates neurotransmitter release in a dosage-dependent manner. EMBO (Eur. Mol. Biol. Organ.) J. 17:127-139.
27. Yang, B., L. Gonzalez, Jr., R. Prekeris, M. Steegmaier, R.J. Advani, and R.H. Scheller. 1999. SNARE interactions are not selective. Implications for membrane fusion specificity. J. Biol. Chem. 274:5649-5653.