Functional probing of the human glucocorticoid receptor steroid- interacting surface by site-directed mutagenesis: Gln-642 plays an important role in steroid recognition and binding

Journal of Biological Chemistry

Volumn 275, Issue 25, 2000, Pages 19041-19049

  Lind, Ulrika ^a,b   Greenidge, Paulette ^e   Gillner, Mikael ^e   Koehler, Konrad F ^e   Wright, Anthony ^c,d   Carlstedt Duke, Jan ^a,b  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b Akaro Bio AB   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

^d SÖDERTÖRN UNIVERSITY   (Sweden)

^e NONE

Author keywords

[No Author keywords available]

Indexed keywords

11BETA HYDROXYPROGESTERONE; CORTICOSTERONE; DEOXYCORTICOSTERONE; DESONIDE; DEXAMETHASONE; FLUDROCORTISONE; GLUCOCORTICOID RECEPTOR; GLYCINE; HYDROCORTISONE; METHIONINE; PREDNISOLONE; THREONINE; TRIAMCINOLONE; TRIAMCINOLONE ACETONIDE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; BINDING SITE; CRYSTAL STRUCTURE; HORMONE RECEPTOR INTERACTION; HUMAN; HUMAN CELL; HYDROPHOBICITY; LIGAND BINDING; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ANIMALS; COS CELLS; GLYCINE; HUMANS; MOLECULAR PROBES; MUTAGENESIS, SITE-DIRECTED; RECEPTORS, GLUCOCORTICOID; STEROIDS;

EID: 0034705588     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M000228200     Document Type: Article

References (43)

1. Evans, R. M. (1988) Science 240, 889-895
2. Mangelsdorf, D. J., Thummel, C., Beato, M., Herrlich, P., Schutz, G., Umesono, K., Blumberg, B., Kastner, P., Mark, M., and Chambon, P. (1995) Cell 83, 835-839
3. Beato, M., Herrlich, P., and Schutz, G. (1995) Cell 83, 851-857
4. Carlstedt-Duke, J., Strömstedt, P.-E., Wrange, O., Bergman, T., Gustafsson, J.-Å., and Jörnvall, H. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 4437-4440
5. Pratt, W. B., Hutchison, K. A., and Scherrer, L. C. (1992) Trends Endocrinol. Metab. 3, 326-333
6. Picard, D., and Yamamoto, K. R. (1987) EMBO J. 6, 3333-3340
7. Hollenberg, S. M., and Evans, R. M. (1988) Cell 55, 899-906
8. Danielsen, M., Northrop, J. P., Jonklaas, J., and Ringold, G. M. (1987) Mol. Endocrinol. 1, 816-822
9. Godowski, P. J., Picard, D., and Yamamoto, K. R. (1988) Science 241, 812-816
10. Hollenberg, S. M., Giguere, V., Segui, P., and Evans, R. M. (1987) Cell 49, 39-46
11. Nolte, R. T., Wisely, G. B., Westin, S., Cobb, J. E., Lambert, M. H., Kurokawa, R., Rosenfeld, M. G., Willson, T. M., Glass, C. K., and Milburn, M. V. (1998) Nature 395, 137-143
12. Renaud, J.-P., Rochel, N., Ruff, M., Vivat, V., Chambon, P., Gronemeyer, H., and Moras, D. (1995) Nature 378, 681-689
13. Bourguet, W., Ruff, M., Chambon, P., Gronemeyer, H., and Moras, D. (1995) Nature 375, 377-382
14. Wagner, R. L., Apriletti, J. W., McGrath, M. E., West, B. L., Baxter, J. D., and Fletterick, R. J. (1995) Nature 378, 690-697
15. Williams, S. P., and Sigler, P. B. (1998) Nature 393, 392-396
16. Brzozowski, A. M., Pike, A. C., Dauter, Z., Hubbard, R. E., Bonn, T., Engström, O., Öhman, L., Greene, G. L., Gustafsson, J.-Å., and Carlquist, M. (1997) Nature 389, 753-758
17. Pike, A. C. W., Brzozowski, A. M., Hubbard, R. E., Bonn, T., Thorsell, A. G., Engström, O., Ljunggren, J., Gustafsson, J.-Å., and Carlquist, M. (1999) EMBO J. 18, 4608-4618
18. Feng, W., Ribeiro, R. C., Wagner, R. L., Nguyen, H., Apriletti, J. W., Fletterick, R. J., Baxter, J. D., Kushner, P. J., and West, B. L. (1998) Science 280, 1747-1749
19. Darimont, B. D., Wagner, R. L., Apriletti, J. W., Stallcup, M. R., Kushner, P. J., Baxter, J. D., Fletterick, R. J., and Yamamoto, K. R. (1998) Genes Dev. 12, 3343-3356
20. Shiau, A. K., Barstad, D., Loria, P. M., Cheng, L., Kushner, P. J., Agard, D. A., and Greene, G. L. (1998) Cell 95, 927-937
21. Mak, H. Y., Hoare, S., Henttu, P. M., and Parker, M. G. (1999) Mol. Cell. Biol. 19, 3895-3903
22. Tanenbaum, D. M., Wang, Y., Williams, S. P., and Sigler, P. B. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 5998-6003
23. Lind, U., Carlstedt-Duke, J., Gustafsson, J.-A., and Wright, A. P. (1996) Mol. Endocrinol. 10, 1358-1370
24. Schule, R., Muller, M., Kaltschmidt, C., and Renkawitz, R. (1988) Science 242, 1418-1420
25. Kunkel, T. A., Roberts, J. D., and Zakour, R. A. (1987) Methods Enzymol. 154, 367-382
26. McClary, J. A., Witney, F., and Geisselsoder, J. (1989) BioTechniques 7, 282-289
27. Womble, D. D. (2000) Methods Mol. Biol. 132, 3-22
28. Sali, A. (1995) Curr. Opin. Biotechnol. 6, 437-451
29. Hollenberg, S. M., Weinberger, C., Ong, E S., Cerelli, G., Oro, A., Lebo, R., Thompson, E. B., Rosenfeld, M. G., and Evans, R. M. (1985) Nature 318, 635-641
30. Singh, U. C., and Kollman, P. A. (1984) J. Computat. Chem. 5, 129-145
31. Brooks, C. L., III, Brunger, A., and Karplus, M. (1985) Biopolymers 24, 843-865
32. Bower, M. J., Cohen, F. E., and Dunbrack, R. L., Jr. (1997) J. Mol. Biol. 267, 1268-1282
33. Mohamadi, F., Richards, N. G. J., Guida, W. C., Liskamp, R., Caufield, C., Chang, G., Hendrickson, T., and Still, W. C. (1990) J. Comput. Chem. 11, 440-467
34. McDonald, D. Q., and Still, W. C. (1992) Tet. Lett. 33, 7743-7746
35. Burling, F. T., and Goldstein, B. M. (1992) J. Am. Chem. Soc. 114, 2313-2320
36. Roaenfield, R. E., Parthasarathy, R., and Dunitz, J. D. (1977) J. Am. Chem. Soc. 99, 4860-4862
37. Burling, F. T., and Goldutein, B. M. (1993) Acta Crystallogr. Sect. B Struct. Sci. 49, 738-744
38. Fagart, J., Wurtz, J. M., Souque, A., Hellallevy, C., Moras, D., and Rafestinoblin, M. E. (1998) EMBO J. 17, 3317-3325
39. Wurtz, J.-M., Bourguet, W., Renaud, J.-P., Vivat, V., Chambon, P., Moras, D., and Gronemeyer, H. (1996) Nat. Struct. Biol. 3, 87-94
40. Wolff, M. E., Baxter, J. D., Kollman, P. A., Lee, D. L., Kuntz, I. D., Bloom, E., Matulich, D. T., and Morris, J. (1978) Biochemistry 17, 3201-3208
41. Verlet, L. (1967) Physiol. Rev. 159, 98-105
42. Ryckaert, J.-P., Ciccotti, G., and Berendsen, H. J. C. (1977) J. Comput. Physiol. 23, 327-341
43. Jorgensen, W. L., Chandrasekhar, J., and Madura, J. D. (1983) J. Chem. Physiol. 79, 926-935