Probing the Escherichia coli transcriptional activator MarA using alanine-scanning mutagenesis: Residues important for DNA binding and activation

Journal of Molecular Biology

Volumn 299, Issue 5, 2000, Pages 1245-1255

  Gillette, William K ^a   Martin, Robert G ^a   Rosner, Judah L ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

AraC; Backbone contacts; Hydrophobic core; Marbox; RNA polymerase

Indexed keywords

HELIX LOOP HELIX PROTEIN; PROTEIN; PROTEIN MAR A; RNA POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; DNA BINDING; ESCHERICHIA COLI; GENE ACTIVATION; GENE SEQUENCE; HYDROGEN BOND; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN STRUCTURE; TRANSCRIPTION REGULATION; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

EID: 0034705326     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3827     Document Type: Article

References (19)

1. Regulation of chromosomally mediated multiple antibiotic resistance: The mar regulon
2. Amino acid-DNA contacts by RhaS: An AraC family transcription activator
3. Determining residue-base interactions between AraC protein and araI DNA
4. Transcription activation by catabolite activator protein (CAP)
5. AraC/XylS family of transcriptional regulators
6. Structural homology between MarA of the AraC family of transcriptional activators and the integrase family of site-specific recombinases
7. Purification and regulatory properties of MarA protein, a transcriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters
8. Alternative packing arrangements in the hydrophobic core of lambda repressor
9. The role of internal packing interactions in determining the structure and stability of a protein
10. Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences
11. Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob
12. Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: Sequence, orientation and spatial relationship to the core promoter
13. Promoter discrimination by the related transcriptional activators, MarA and SoxS: Differential regulation by differential binding
14. Binding site recognition by Rns, a virulence regulator in the AraC family
15. How AraC interacts specifically with its target DNAs
16. A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator
17. Interdependence of the position and orientation of SoxS binding sites in the transcriptional activation of the class I subset of Escherichia coli superoxide-inducible promoters
18. Mutations leading to constitutive expression from the TOL plasmid meta-cleavage pathway operon are located at the C-terminal end of the positive regulator protein XylS