In vivo importance of actin nucleotide exchange catalyzed by profilin

Journal of Cell Biology

Volumn 150, Issue 4, 2000, Pages 895-903

  Wolven, A K ^a   Belmont, L D ^a   Mahoney, N M ^a   Almo, S C ^a   Drubin, D G ^a  

^a University of California   (United States)

Author keywords

ATP; Cofilin; Cytoskeleton; Filament turnover; Yeast

Indexed keywords

ACTIN; NUCLEOTIDE; PHOSPHATIDYLINOSITIDE; PROFILIN; PROLINE DERIVATIVE;

ACTIN FILAMENT; ARTICLE; CATALYSIS; CYTOSKELETON; ENDOCYTOSIS; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN METABOLISM; PROTEIN MODIFICATION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

ACTINS; CONTRACTILE PROTEINS; KINETICS; MICROFILAMENT PROTEINS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; POINT MUTATION; PROFILINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

SACCHAROMYCES CEREVISIAE;

EID: 0034698783     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.4.895     Document Type: Article

References (36)

1. In vivo functions of actin-binding proteins
2. The yeast V159N actin mutant reveals roles for actin dynamics in vivo
3. A change in actin conformation associated with filament instability after Pi release
4. New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites
5. Interaction of actin monomers with Acanthamoeba actophorin (ADF/cofilin) and profilin
6. Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells
7. Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast
8. Synergy between actin depolymerizing factor/cofilin and profilin in increasing actin filament turnover
9. Structure determination and characterization of Saccharomyces cerevisiae profilin
10. Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis
11. An actin monomer binding activity localizes to the carboxyl-terminal half of the Saccharomyces cerevisiae cyclase-associated protein
12. Mechanism of the interaction of human platelet profilin with actin
13. Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer sequestering protein
14. Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells
15. Bni1P and Bnr1P: Downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae
16. Profilin promotes barbed-end actin filament assembly without lowering the critical concentration
17. Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae
18. Cofilin promotes rapid actin filament turnover in vivo
19. Essential functions and actin-binding surfaces of yeast cofilin revealed by systematic mutagenesis
20. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
21. Profilin as a potential mediator of membrane-cytoskeleton communication
22. Acanthamoeba profilin interacts with G-actin to increase the rate of exchange of actin-bound adenosine 5'-triphosphate
23. How profilin promotes actin filament assembly in the presence of thymosin β-4
24. Binding of divalent cation and nucleotide to G-actin in the presence of profilin
25. Role of nucleotide exchange and hydrolysis in the function of profilin in actin assembly
26. Structure of actin binding proteins: Insights about function at atomic resolution
27. Polarization of cell growth in yeast
28. Aip1p interacts with cofilin to disassemble actin filaments
29. The bulk of unpolymerized actin in Xenopus egg extracts is ATP-bound
30. Affinity chromatography-based purification of profilin: Actin
31. The structure of crystalline profilin-beta-actin
32. Impact of profilin on actin-bound nucleotide exchange and actin polymerization dynamics
33. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
34. Interactions of Acanthamoeba profilin with actin and nucleotides bound to actin
35. FH proteins as cytoskeletal organizers