Membrane topogenesis of a type I signal-anchor protein, mouse synaptotagmin II, on the endoplasmic reticulum

Journal of Cell Biology

Volumn 150, Issue 4, 2000, Pages 719-729

  Kida, Yuichiro ^a   Sakaguchi, Masao ^a   Fukuda, Mitsunori ^b   Mikoshiba, Katsuhiko ^b   Mihara, Katsuyoshi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

Author keywords

Membrane protein; Membrane topology; Protein translocation; Signal anchor sequence; Translocon

Indexed keywords

POLYPEPTIDE; SYNAPTOTAGMIN;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; ENDOPLASMIC RETICULUM; MEMBRANE TRANSPORT; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN TARGETING; PROTEIN TRANSPORT; RIBOSOME;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CONSENSUS SEQUENCE; COS CELLS; ENDOPLASMIC RETICULUM; GLYCOSYLATION; INTRACELLULAR MEMBRANES; MEMBRANE PROTEINS; MICE; MICROSOMES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NERVE TISSUE PROTEINS; PEPTIDE FRAGMENTS; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RABBITS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SYNAPTOTAGMIN II; TRANSFECTION;

EID: 0034698758     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.4.719     Document Type: Article

References (55)

1. The amino-terminal 29 amino acids of cytochrome P450 2C1 are sufficient for retention in the endoplasmic reticulum
2. Immunoprecipitation of proteins from cell-free translations
3. A two-step recognition of signal sequences determines the translocation efficiency of proteins
4. Biogenesis of polytopic membrane proteins: Membrane segments assemble within translocation channels prior to membrane integration
5. Fatty acylation of synaptotagmin in PC12 cells and synaptosomes
6. Transmembrane orientation of signal-anchor proteins is affected by the folding state but not the size of the N-terminal domain
7. Isolation of intracellular membranes by means of sodium carbonate treatment: Application to endoplasmic reticulum
8. The function of inositol high polyphosphate binding proteins
9. Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II
10. Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X
11. A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms
12. Synaptotagmin II. A novel differentially distributed form of synaptotagmin
13. Membrane protein biosynthesis - All sewn up?
14. Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA
15. A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane
16. Membrane topology of NADPH-cytochrome P450 reductase on the endoplasmic reticulum
17. Rapid and efficient site-specific mutagenesis without phenotypic selection
18. Structural requirements for interruption of protein translocation across rough endoplasmic reticulum membrane
19. Systematic analysis of stop-transfer sequence for microsomal membrane
20. 2-dependent and -independent activities of neural and non-neural synaptotagmins
21. Uncoupling hydrophobicity and helicity in transmembrane segments. Alpha-helical propensities of the amino acids in non-polar environments
22. The 70 carboxyl-terminal amino acids of nascent secretory proteins are protected from proteolysis by the ribosome and the protein translocation apparatus of the endoplasmic reticulum membrane
23. Protein translocation: Tunnel vision
24. Signals for the incorporation and orientation of cytochrome P450 in the endoplasmic reticulum membrane
25. A turn propensity scale for transmembrane helices
26. An investigation of the role of transmembrane domains in Gotgi protein retention
27. The transmembrane region of microsomal cytochrome P450 identified as the endoplasmic reticulum retention signal
28. Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane
29. The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase
30. Calcium-dependent oligomerization of synaptotagmins I and II. Synaptotagmins I and II are localized on the same synaptic vesicle and heterodimerize in the presence of calcium
31. Assessment of topogenic functions of anticipated transmembrane segments of human Band 3
32. Forced transmembrane orientation of hydrophilic polypeptide segments in multispanning membrane proteins
33. Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain
34. Role of NH2-terminal positively charged residues in establishing membrane protein topology
35. A mutant cytochrome b5 with a lengthened membrane anchor escapes from the endoplasmic reticulum and reaches the plasma membrane
36. Domain structure of synaptotagmin (p65)
37. Mapping the ends of transmembrane segments in a polytopic membrane protein
38. Transmembrane domain-dependent sorting of proteins to the ER and plasma membrane in yeast
39. Mutational analysis of signal-anchor and stop-transfer sequences in membrane proteins
40. A short amino-terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence
41. Mitochondrial porin can be translocated across both endoplasmic reticulum and mitochondrial membranes
42. Functions of signal and signal-anchor sequences are determined by the balance between the hydrophobic segment and the N-terminal charge
43. The amino-terminal structures that determine topological orientation of cytochrome P-450 in microsomal membrane
44. Synaptotagmins: More isoforms than functions?
45. Synaptotagmins: C2-domain proteins that regulate membrane traffic
46. The cytoplasmic and N-terminal transmembrane domains of cytochrome P450 contain independent signals for retention in the endoplasmic reticulum
47. Multiple palmitoylation of synaptotagmin and the t-SNARE SNAP-25
48. The signal peptide
49. Multiple determinants direct the orientation of signal-anchor proteins: The topogenic role of the hydrophobic signal domain
50. Properties of N-terminal tails in G-protein coupled receptors: A statistical study
51. Preparation of microsomal membranes for cotranslational protein translocation
52. Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane
53. A nascent secretory protein may traverse the ribosome/endoplasmic reticulum translocase complex as an extended chain
54. The transmembrane domain of a carboxyl-terminal anchored protein determines localization to the endoplasmic reticulum