Use of a new label, 13C = 18O, in the determination of a structural model of phospholamban in a lipid bilayer. Spatial restraints resolve the ambiguity arising from interpretations of mutagenesis data

Journal of Molecular Biology

Volumn 300, Issue 4, 2000, Pages 677-685

  Torres, Jaume ^a   Adams, Paul D ^b   Arkin, Isaiah T ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Infrared spectroscopy; Ion channel; Molecular dynamics; Phospholamban

Indexed keywords

CARBON 13; OXYGEN 18; PHOSPHOLAMBAN; AMIDE; CALCIUM BINDING PROTEIN; CARBON; OXYGEN; PEPTIDE FRAGMENT;

ARTICLE; CHEMICAL BOND; INFRARED SPECTROSCOPY; LIPID BILAYER; MOLECULAR DYNAMICS; MOLECULAR MODEL; MUTAGENESIS; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN STRUCTURE; SIMULATION; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; GENETICS; METABOLISM; PROTEIN SECONDARY STRUCTURE; VIBRATION;

AMIDES; CALCIUM-BINDING PROTEINS; CARBON ISOTOPES; COMPUTER SIMULATION; LIPID BILAYERS; MODELS, MOLECULAR; MUTAGENESIS; OXYGEN ISOTOPES; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, SECONDARY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; VIBRATION;

EID: 0034697895     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3885     Document Type: Article

References (32)

1. Computational searching and mutagenesis suggest a structure for the pentameric transmembrane domain of phospholamban
2. Structural organization of the pentameric transmembrane alpha-helices of phospholamban, a cardiac ion chann
3. Structural perspectives of phospholamban, a helical transmembrane pentamer
4. Site-directed dichroism as a method for obtaining rotational and orientational constraints for oriented polymers
5. Mapping the lipid-exposed surfaces of membrane proteins
6. Structural model of the phospholamban ion channel complex in phospholipid membranes
7. 2+ pump and phospholamban in Spodoptera frugiperda (Sf21) cells reveals new insights on ATPase regulation
8. Crystallography and NMR system: A new software system for macromolecular structure determination
9. Examination of the secondary structure of proteins by deconvolved FTIR spectra
10. Monomeric phospholamban overexpression in transgenic mouse hearts
11. Pentameric assembly of phospholamban facilitates inhibition of cardiac function in vivo
12. The expression of canine cardiac phospholamban in heterologous systems
13. Mutation and phosphorylation change the oligomeric structure of phospholamban in lipid bilayers
14. Expression and site-specific mutagenesis of phospholamban. Studies of residues involved in phosphorylation and pentamer formation
15. Using experimental information to produce a model of the transmembrane domain of the ion channel phospholamban
16. The OPLS potential function for proteins, energy minimization for crystals of cyclic peptides and crambin
17. Cysteine reactivity and oligomeric structures of phospholamban and its mutants
18. 2+-ATPase isoform of cardiac sarcoplasmic reticulum
19. 2+-ATPase through intramembrane interactions
20. Phospholamban inhibitory function is activated by depolymerization
21. Effects of adenosine 3',5'-monophosphate dependent protein kinase on sarcoplasmic reticulum oslated from cardiac and slow and fast contracting skeletal muscles
22. 2+-selective channels in lipid bilayers
23. + channel
24. Phospholamban: Protein structure, mechanism of action, and role in cardiac function
25. A leucine zipper stabilizes the pentameric membrane domain of phospholamban and forms a coiled-coil pore structure
26. Secondary structure of detergent-solubilized phospholamban, a phosphorylatable, oligomeric protein of cardiac sarcoplasmic reticulum
27. Secondary structure and orientation of phospholamban reconstituted in supported bilayers from polarized attenuated total reflection FTIR spectroscopy
28. 2+-ATPase of sarcoplasmic reticulum
29. Infrared dichroism and molecular conformation of α-form poly-γ-benzyl-L-glutamate
30. Phosphorylation-induced mobility shift in phospholamban in sodium dodecyl sulfate-polyacrylamide gels. Evidence for a protein structure consisting of multiple identical phosphorylatable subunits
31. Standard FMOC protocols