Double fluorescence resonance energy transfer to explore multicomponent binding interactions: A case study of DNA mismatches

Chemical Communications

Volumn , Issue 12, 2000, Pages 1043-1044

  Horsey, Imogen ^a   Furey, W Scott ^a   Harrison, Joseph G ^a   Osborne, Mark A ^a   Balasubramanian, Shankar ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BASE MISPAIRING; COMPLEX FORMATION; DNA BINDING; DNA SEQUENCE; FLUORESCENCE ANALYSIS; FLUOROMETRY;

EID: 0034697840     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b002540m     Document Type: Article

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9. 9mers were all of the same base composition so that melt temperatures were similar for the three oligonucleotides.
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11. Preliminary studies showed FRET was most efficient when the two fluorophores were separated by a distance of seven bases.
12. A:A in the central region of a duplex have the greatest effect on duplex stability: see, for example: F. Aboul-ela, D. Koh and J. J. Tinoco, Nucleic Acids Res., 1985, 13, 4811; B. L. Gaffney and R. A. Jones, Biochemistry, 1989, 5881.
13. A:A in the central region of a duplex have the greatest effect on duplex stability: see, for example: F. Aboul-ela, D. Koh and J. J. Tinoco, Nucleic Acids Res., 1985, 13, 4811; B. L. Gaffney and R. A. Jones, Biochemistry, 1989, 5881.
14. a-c are the observed emission intensities of the sample of interest at wavelengths a-c; x, y and z are unknowns representing the relative contributions to the fluorescence signal by ROX, FAM and TMR, respectively, to the total emission.