Azotobacter vinelandii nitrogenases containing altered MoFe proteins with substitutions in the FeMo-cofactor environment: Effects on the catalyzed reduction of acetylene and ethylene

Biochemistry

Volumn 39, Issue 11, 2000, Pages 2970-2979

  Fisher, Karl ^c   Dilworth, Michael J ^a,c   Kim, Chul Hwan ^b,c   Newton, William E ^c  

^a MURDOCH UNIVERSITY   (Australia)

^b UNIVERSITY OF GEORGIA   (United States)

^c VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLENE; ADENOSINE TRIPHOSPHATE MAGNESIUM; ASPARAGINE; BACTERIAL PROTEIN; ETHANE; ETHYLENE; GLUTAMINE; HISTIDINE; LYSINE; NITROGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; AZOTOBACTER VINELANDII; CATALYSIS; ELECTRON TRANSPORT; ENZYME ANALYSIS; HYDROLYSIS; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTON TRANSPORT; REDUCTION; STEREOCHEMISTRY; STEREOSPECIFICITY;

ACETYLENE; AMINO ACID SUBSTITUTION; AZOTOBACTER VINELANDII; CATALYSIS; ENZYME ACTIVATION; ENZYME INHIBITORS; ETHYLENES; KINETICS; MOLYBDOFERREDOXIN; MUTAGENESIS, SITE-DIRECTED; NITROGENASE; OXIDATION-REDUCTION; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0034696641     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi992092e     Document Type: Article

References (56)

1. Burns, R. C., and Bulen, W. A. (1965) Biochim. Biophys. Acta 105, 437-445.
2. Dilworth, M. J. (1966) Biochim. Biophys Acta 127, 285-294.
3. Hwang, J. C., and Burris, R. H. (1972) Biochim. Biophys. Acta 283, 339-350.
4. Davis, L. C., Henzl, M. T., Burris, R. H., and Orme-Johnson, W. H. (1979) Biochemistry 18, 4860-4869.
5. Ashby, G. A., Dilworth, M. J., and Thorneley, R. N. F. (1987) Biochem. J. 247, 547-554.
6. Hardy, R. W. F., Holsten, R. D., Jackson, E. K., and Burns, R. C. (1968) Plant Physiol 43, 1185-1207.
7. Kelly, M. (1969) Biochim. Biophys. Acta 191, 527-540.
8. Lin-Vein, D., Fateley, W. G., and Davis, L. C. (1989) Appl. Environ. Microbiol. 55, 354-359.
9. Hardy, R. W. F., Knight, E., Jr., and D'Eustachio, A. J. (1965) Biochem. Biophys. Res. Commun. 20, 539-544.
10. Bulen, W. A., Burns, R. C., LeComte, J. R., and Hinkson, J. (1965) in Non-Heme Iron Proteins: Role in Energy Conversion (San Pietro, A., Ed.) pp 261-274, Antioch Press, Yellow Springs, OH.
11. Georgiadis, M. M., Komiga, H., Chakrabarti, P., Woo, D., Kornuc, J. J., and Rees, D. C. (1992) Science 257, 1653-1659.
12. Kim, J., and Rees, D. C. (1992) Science 257, 1677-1682.
13. Kim, J., and Rees, D. C. (1992) Nature 360, 553-560.
14. Kim, J., Woo, D., and Rees, D. C. (1993) Biochemistry 32, 7104-7115.
15. Chan, M. K., Kim, J., and Rees, D. C. (1993) Science 260, 792-794.
16. Bolin, J. T., Campobasso, N., Muchmore, S. W., Minor, W., Morgan, V. T., and Mortenson, L. E. (1993) in New Horizons in Nitrogen Fixation (Palacios, R., Mora, J., and Newton, W. E., Eds.) pp 89-94, Kluwer Academic Publishers, Boston.
17. Bolin, J. T., Campobasso, N., Muchmore, S. W., Morgan, V. T., and Mortenson, L. E. (1993) in Molybdenum Enzymes, Cofactors and Model Systems (Stiefel, E. I., Coucouvanis, D., and Newton, W. E., Eds.) pp 186-195, American Chemical Society, Washington, DC.
18. Bolin, J. T., Ronco, A. E., Morgan, T. V., Mortenson, L. E., and Xuong, N.-H. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 1078-1082.
19. Shah, V. K., and Brill, W. J. (1979) Proc. Natl. Acad. Sci. U.S.A. 78, 3249-3253.
20. Hawkes, T. R., McLean, P. A., and Smith, B. E. (1984) Biochem. J. 217, 317-321.
21. Scott, D. J., May, H. D., Newton, W. E., Brigle, K. E., and Dean, D. R. (1990) Nature 343, 188-190.
22. Kim, C.-H., Newton, W. E., and Dean, D. R. (1995) Biochemistry 34, 2798-2808.
23. Brigle, K. E., Newton, W. E., and Dean, D. R. (1985) Gene 37, 37-44.
24. Brigle, K. E., Setterquist, R. A., Dean, D. R., Cantwell, J. S., Weiss, M. C. and Newton, W. E. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 7066-7069.
25. Kent, H. M., Ioannides, I., Gormal, C., Smith, B. E., and Buck, M. (1989) Biochem. J. 264, 257-264.
26. Dean, D. R., Scott, D. J., and Newton, W. E. (1990) in Nitrogen Fixation: Achievements and Objectives (Gresshoff, P. M., Roth, L. E., Stacey, G., and Newton, W. E., Eds.), pp 95-102, Chapman & Hall, New York.
27. Dean, D. R., Setterquist, R. A., Brigle, K. E., Scott, D. J., Laird, N. F., and Newton, W. E. (1990) Mol. Microbiol. 4, 1505-1512.
28. Scott, D. J., Dean, D. R., and Newton, W. E. (1992) J. Biol. Chem. 267, 20002-20010.
29. Dean, D. R., and Jacobson, M. R. (1992) in Biological Nitrogen Fixation (Stacey, G., Burris, R. H., and Evans, H. J., Eds.) pp 763-834, Chapman & Hall, New York.
30. Newton, W. E., and Dean, D. R. (1993) in Molybdenum Enzymes, Cofactors and Model Systems (Stiefel, E. I., Coucouvanis, D., and Newton, W. E., Eds.) pp 216-230. ACS Symposium Series No. 535, American Chemical Society, Washington, DC.
31. Howard, J. B., and Rees, D. C. (1996) Chem. Rev. 96, 2965-2982.
32. Hoover, T. R., Robertson, A. D., Cerny, R. L., Hayes, R. N., Imperial, J., Shah, V., and Ludden, P. W. (1987) Nature 329, 855-857.
33. DeRose, V. J., Kim, C.-H., Newton, W. E., Dean, D. R., and Hoffman, B. M. (1995) Biochemistry 34, 2809-2814.
34. Dilworth, M. J., Fisher, K., Kim, C.-H., and Newton, W. E. (1998) Biochemistry 37, 17495-17505.
35. Strandberg, G. W., and Wilson, P. W. (1968) Can. J. Microbiol. 14, 25-31.
36. Laemmli, U.K. (1970) Nature 227, 680-685.
37. Lowry, O. H, Rosebrough, N. J., Fair, A. L., and Randall, R. J. (1951) J. Biol. Chem. 193, 265-275.
38. Ennor, A. H. (1957) Methods Enzymol. 3, 850-856.
39. Dilworth, M. J., Eldridge, M. E., and Eady, R. R. (1992) Anal. Biochem. 207, 6-10.
40. Dilworth, M. J., Eady, R. R., Robson, R. L., and Miller, R. W. (1987) Nature 327, 167-168.
41. Dilworth, M. J., Eady, R. R., and Eldridge, M. E. (1988) Biochem. J. 249, 745-751.
42. Schneider, K., Muller, A., Schramm, U., and Klipp, W. (1991) Eur. J. Biochem. 195, 653-661.
43. Stiefel, E. I. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 988-992.
44. McKenna, C. E., McKenna, M.-C., and Huang, C. W. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4773-4777.
45. Hawkes, T. R., Lowe, D. J., and Smith, B. E. (1983) Biochem. J. 211, 495-497.
46. Burgess, B. K., and Lowe, D. J. (1996) Chem. Rev. 96, 2983-3011.
47. Lowe, D. J., Fisher, K., and Thorneley, R. N. F. (1990) Biochem. J. 272, 621-625.
48. Thorneley, R. N. F., Ashby, G. A., Julius, C., Hunter, J. L., and Webb, M. R. (1991) Biochem. J. 277, 735-741.
49. Li, J.-G., Burgess, B. K., and Corbin, J. L. (1982) Biochemistry 21, 4393-4402.
50. Nakamura, A. and Otsuka, S. (1972) J. Am. Chem. Soc. 94, 1886-1894.
51. Alt, H. G., and Eicher, M. E. (1982) Angew. Chem., Int. Ed. Engl. 21, 78-79.
52. Kashef, N., and Richards, R. L. (1989) J. Organomet. Chem. 365, 309-315.
53. Richards, R. L. (1991) in Biology and Biochemistry of Nitrogen Fixation (Dilworth, M. J., and Glenn, A. R., Eds.) pp 58-75, Elsevier Science Publishers, Amsterdam, The Netherlands.
54. Kraulis, P. (1991) J. Appl. Crystallogr. 24, 946-950.
55. Christie, P. D., Lee, H.-I., Cameron, L. M., Hales, B. J., Orme-Johnson, W. H., and Hoffman, B. M. (1996) J. Am. Chem. Soc. 118, 8707-8709.
56. Shen, J., Dean, D. R., and Newton, W. E. (1997) Biochemistry 36, 4884-4894.