Colorimetric determination of inhibition of hematopoietic progenitor cells in soft agar

Journal of Immunological Methods

Volumn 244, Issue 1-2, 2000, Pages 49-58

  Horowitz, Daniel ^a   King, Andrew G ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Colony formation; Growth factor; Hematopoiesis; MTT

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AGAR; COLONY STIMULATING FACTOR 1; FLUOROURACIL; GAMMA INTERFERON; GANCICLOVIR; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; TRANSFORMING GROWTH FACTOR BETA1; ZIDOVUDINE;

ANIMAL CELL; ARTICLE; COLONY FORMATION; COLONY FORMING UNIT; COLORIMETRY; CONTROLLED STUDY; FEMALE; HEMATOPOIESIS; IC 50; MOUSE; NONHUMAN; OPTICAL DENSITY; PRECURSOR CELL; PRIORITY JOURNAL;

ANIMAL; ANTIMETABOLITES; ANTIMETABOLITES, ANTINEOPLASTIC; ANTIVIRAL AGENTS; COLONY-FORMING UNITS ASSAY; COLONY-STIMULATING FACTORS; COLORIMETRY; COMPARATIVE STUDY; CYTARABINE; CYTOKINES; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; DYES; FEMALE; FLUOROURACIL; GANCICLOVIR; GROWTH INHIBITORS; HEMATOPOIETIC STEM CELLS; HUMAN; INHIBITORY CONCENTRATION 50; MICE; MICE, INBRED C57BL; MICE, INBRED DBA; TETRAZOLIUM SALTS; THIAZOLES; ZIDOVUDINE;

MURINAE;

EID: 0034693433     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00253-2     Document Type: Article

References (27)

1. Evaluation of MTT assay in agarose for chemosensitivity testing of human cancers: comparison with MTT assay
2. Spectrum of biological activity of interferons. [Review] [324 refs]
3. The growth of mouse bone marrow cells in vitro
4. Development and application of in vitro models of hematopoiesis to drug development
5. In vitro potency of inhibition by antiviral drugs of hematopoietic progenitor colony formation correlates with exposure at hemotoxic levels in human immunodeficiency virus-positive humans
6. Clinical pharmacodynamics of anticancer drugs: a basis for extending the concept of dose-intensity
7. Myelosuppressive effects of antineoplastic chemotherapy
8. Physiology and function of the haemopoietic microenvironment
9. The haemopoietic stem cell: properties and control mechanisms
10. Growth inhibitors in haemopoiesis and leukaemogenesis
11. In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitors cells
12. Initiation of growth inhibition by TGF beta 1 is unlikely to occur in G1
13. Conclusions of a national multicenter intercomparative study of in vitro cultures of human hematopoietic progenitors
14. A cellular model for drug interactions on hematopoiesis-the use of human umbilical cord blood progenitors as a model for the study of drug-related myelosuppression of normal hematopoiesis
15. Quality assurance of CFU-GM assays: inter-laboratory variation despite standard reagents
16. The effects of cancer chemotherapeutic agents on normal hematopoietic precursor cells: a review. [Review] [206 refs]
17. Hemopoietic growth factors: a review
18. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
19. Two forms of transforming growth factor-beta distinguished by multipotential haematopoietic progenitor cells
20. Stroma-dependent hematolymphopoietic stem cells. [Review] [130 refs]
21. Pleiotropic effects of transforming growth factor-beta on cells of the immune system. [Review] [56 refs]
22. Acute toxic effects of 3'-azido-3'-deoxythymidine (AZT) on normal and regenerating murine hematopoiesis
23. Haemopoietic and osteogenic toxicity testing in vitro using murine bone marrow cultures
24. Spectrophotometric determination of clonogenic capacity of leukemic cells in a semisolid microtiter culture system
25. Hematopoietic progenitors and synergism of interferon-gamma and stem cell factor. [Review] [71 refs]
26. The effects of 5-fluorouracil on hematopoiesis: studies of murine megakaryocyte-CFC, granulocyte-macrophage-CFC, and peripheral blood cell levels