Detection of mutation of the p53 gene with high sensitivity by fluorescence-based PCR-SSCP analysis using low-pH buffer and an automated DNA sequencer in a large number of DNA samples

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 452, Issue 1, 2000, Pages 83-90

  Makino, Reiko ^a   Kaneko, Kazuhiro ^a   Kurahashi, Toshinori ^a   Matsumura, Takuya ^a   Mitamura, Keiji ^a  

^a SHOWA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Clinical diagnosis; Colorectal carcinoma; Esophageal carcinoma; Fluorescence based PCR SSCP; Hepatocellular carcinoma; Low pH buffer; Mutation; p53; PCR SSCP

Indexed keywords

BUFFER; DNA; DNA FRAGMENT; GLYCEROL; POLYACRYLAMIDE GEL; PROTEIN P53;

ARTICLE; AUTOMATION; CANCER DIAGNOSIS; COLORECTAL CARCINOMA; CONTROLLED STUDY; DNA POLYMORPHISM; DNA SEQUENCE; ESOPHAGUS CARCINOMA; EXON; FLUORESCENCE; GENE MUTATION; HUMAN; HUMAN TISSUE; LIVER CELL CARCINOMA; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SAMPLE; SINGLE STRAND CONFORMATION POLYMORPHISM; TUMOR BIOPSY; TUMOR SUPPRESSOR GENE;

DNA MUTATIONAL ANALYSIS; DNA, NEOPLASM; FLUORESCENCE; HUMANS; HYDROGEN-ION CONCENTRATION; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SENSITIVITY AND SPECIFICITY; TEMPERATURE; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0034691830     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(00)00056-7     Document Type: Article

References (23)

1. Hayashi K., Kukita Y., Inazuka M., Tahira T. Single-strand conformation polymorphism analysis. Forrest S.M., Cotton R.G.H. Mutation Detection: A Practical Approach. 1998;7-24 Oxford Univ. Press, Oxford.
2. Orita M., Suzuki Y., Sekiya T., Hayashi K. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics. 5:1989;874-879.
3. Ushijima T., Hosoya Y., Suzuki T., Sofuni T., Sugimura T., Nagao M.T. A rapid method for detection of mutations in the lacI gene using PCR-single strand conformation polymorphism analysis: demonstration of its high sensitivity. Mutat. Res. 334:1995;283-292.
4. Hayashi K. PCR-SSCP: a method for detection of mutations. GATA. 9:1992;73-79.
5. Makino R., Yazyu H., Kishimoto Y., Sekiya T., Hayashi K. F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. PCR Methods Appl. 2:1992;10-13.
6. Kukita Y., Tahira T., Sommer S.S., Hayashi K. SSCP analysis of long DNA fragments in low pH gel. Hum. Mutat. 10:1997;400-407.
7. Harris C.C., Hollstein M. Clinical implications of the p53 tumor-suppressor gene. New Engl. J. Med. 329:1993;1318-1327.
8. Hollestein M., Sidransky D., Vogelstein B., Harris C.C. p53 mutations in human cancers. Science. 253:1991;49-53.
9. Levine A.J. p53, The cellular gatekeeper for growth and division. Cell. 88:1997;323-331.
10. Hainaut P., Hernandez T., Robinson A., Rodriguez-Tome P., Flores T., Hollstein M., Harris C.C., Montesano R. IARC Database of p53 gene mutations in human tumors and cell lines: updated compilation, revised formats and new visualization tools. Nucleic Acids Res. 26:1998;205-213.
11. Fearon E.R., Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 61:1990;759-767.
12. Mashiyama S., Murakami Y., Yoshimoto T., Sekiya T., Hayashi K. Detection of p53 gene mutations in human brain tumors by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene. 6:1991;1313-1318.
13. Suzuki Y., Sekiya T., Hayashi K. Allele-specific polymerase chain reaction: a method for amplification and sequence determination of a single component among a mixture of sequence variants. Anal. Biochem. 192:1991;82-84.
14. Hamelin R., Laurent-Puig P., Olschwang S., Jego N., Asselain B., Remvikos Y., Girodet J., Salmon R.J., Thomas G. Association of p53 mutations with short survival in colorectal cancer. Gastroenterology. 106:1994;42-48.
15. Hayashi H., Sugio K., Matsumata T., Adachi E., Takenaka K., Sugimachi K. The clinical significance of p53 gene mutation in hepatocellular carcinomas from Japan. Hepatology. 22:1995;1702-1707.
16. Oda T., Tsuda H., Scarpa A., Sakamoto M., Hirohashi S. p53 gene mutation spectrum in hepatocellular carcinoma. Cancer Res. 52:1992;6358-6364.
17. Shi S.T., Yang G.Y., Wang L.D., Xue Z., Feng B., Ding W., Xing E.P., Yang C.S. Role of p53 gene mutations in human esophageal carcinogenesis: results from immunohistochemical and mutation analyses of carcinomas and nearby non-cancerous lesions. Carcinogenesis. 20:1999;591-597.
18. Hsu I.C., Metcalf R.A., Sun T., Welsh J.A., Wang N.J., Harris C.C. Mutational hotspot in the p53 gene in human hepatocellular carcinomas. Nature. 350:1991;427-428.
19. Bressac B., Kew M., Wands J., Ozturk M. Selective G to T mutations of p53 gene in hepatocellular carcinoma from southern Africa. Nature. 350:1991;429-431.
20. Iwahana H., Yoshimoto K., Mizusawa N., Kudo E., Itakura M. Multiple fluorescence-based PCR-SSCP analysis. Biotechniques. 16:1994;296-297.
21. Hebenbrock K., Williams P.M., Karger B.L. Single-strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection. Electrophoresis. 16:1995;1429-1436.
22. Inazuka M., Wenz H.M., Sakabe M., Tahira T., Hayashi K. A streamlined mutation detection system: multicolor post-PCR fluorescence labeling and single-strand conformational polymorphism analysis by capillary electrophoresis. Genome Res. 7:1997;1094-1103.
23. Wenz H.M., Ramachandra S., O'Connell C.D., Atha D.H. Identification of known p53 point mutations by capillary electrophoresis using unique mobility profiles in a blinded study. Mutat. Res. 382:1998;121-132.