Engineering CHO cells to overexpress a secreted reporter protein upon induction from mouse mammary tumor virus promoter

Biotechnology and Bioengineering

Volumn 67, Issue 2, 2000, Pages 134-140

  James, Rohaizah I ^a   Elton, John P ^a   Todd, Paul ^a   Kompala, Dhinakar S ^a  

^a UCB 450   (United States)

Author keywords

CHO cells; Dexamethasone; Gene expression; Glucocorticoid receptor; Glucocorticoid response elements; Mammalian cells; MMTV promoter; Overexpression; Secreted alkaline phosphatase

Indexed keywords

ANIMAL CELL CULTURE; BATCH CELL CULTURE; BIOREACTORS; ENZYME KINETICS; GENES; HORMONES; PHYSIOLOGICAL MODELS;

DEXAMETHASONE; GENE EXPRESSION; GLUCOCORTICOID RECEPTORS; GLUCOCORTICOID RESPONSE ELEMENTS; MOUSE MAMMARY TUMOR VIRUS PROMOTER; SECRETED ALKALINE PHOSPHATASE (SEAP);

CLONING;

ANIMAL CELL; ARTICLE; CHO CELL; EXPRESSION VECTOR; GENE OVEREXPRESSION; MOUSE MAMMARY TUMOR ONCOVIRUS; NONHUMAN; PROMOTER REGION; REPORTER GENE;

ALKALINE PHOSPHATASE; ANIMALS; CHO CELLS; CRICETINAE; CULTURE MEDIA; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; MAMMARY TUMOR VIRUS, MOUSE; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECEPTORS, GLUCOCORTICOID; RECOMBINANT PROTEINS; SIMIAN VIRUS 40; TRANSFECTION;

EID: 0034688317     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000120)67:2<134::AID-BIT2>3.0.CO;2-V     Document Type: Article

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