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0342644725
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note
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The partial hlscU gene was a generous gift from Dr. C-C. Liew (U. Toronto). Excluding a mitochondrial targeting sequence, the mature protein has a predicted N-terminal sequence YHKKYVDHYENDPR, where R is the first residue of the partial hlscU. The numbering for hlscU in this work assumes predicted N-terminal Tyr.
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17
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0343950740
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note
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hlscu was cloned into peT-28b (Novagen) and expressed with an N-terminal 6xHis-tag. D37A hlscU as well as C35A-D37A, C61A-D37A, C96A-D37A, and C104A-D37A hlscUs were made using QuickChange Mutagenesis (Stratagene). All mutations were confirmed by sequencing.
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18
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0343078894
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note
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Native hlscU was purified by Ni-NTA chromatography. D37A hlscU was purified by Ni-NTA, P-11, and Superose-12 chromatography.
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21
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0028047783
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0343078888
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note
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57Co source from Isotope Products Laboratory.
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23
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0343514977
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note
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max = 20 s) for a total of ∼40 min/scan. Eight scans were examined to confirm the absence of artifacts or photoreduction and then averaged.
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24
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0343078890
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note
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20b calibrated by fitting data for models of known structure.
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25
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15844409181
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