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Volumn 122, Issue 28, 2000, Pages 6805-6806

A mutant human IscU protein contains a stable [2Fe-2S]2+ center of possible functional significance [21]

Author keywords

[No Author keywords available]

Indexed keywords

IRON; NITROGENASE; SULFUR;

EID: 0034686765     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja000800+     Document Type: Letter
Times cited : (78)

References (29)
  • 16
    • 0342644725 scopus 로고    scopus 로고
    • note
    • The partial hlscU gene was a generous gift from Dr. C-C. Liew (U. Toronto). Excluding a mitochondrial targeting sequence, the mature protein has a predicted N-terminal sequence YHKKYVDHYENDPR, where R is the first residue of the partial hlscU. The numbering for hlscU in this work assumes predicted N-terminal Tyr.
  • 17
    • 0343950740 scopus 로고    scopus 로고
    • note
    • hlscu was cloned into peT-28b (Novagen) and expressed with an N-terminal 6xHis-tag. D37A hlscU as well as C35A-D37A, C61A-D37A, C96A-D37A, and C104A-D37A hlscUs were made using QuickChange Mutagenesis (Stratagene). All mutations were confirmed by sequencing.
  • 18
    • 0343078894 scopus 로고    scopus 로고
    • note
    • Native hlscU was purified by Ni-NTA chromatography. D37A hlscU was purified by Ni-NTA, P-11, and Superose-12 chromatography.
  • 22
    • 0343078888 scopus 로고    scopus 로고
    • note
    • 57Co source from Isotope Products Laboratory.
  • 23
    • 0343514977 scopus 로고    scopus 로고
    • note
    • max = 20 s) for a total of ∼40 min/scan. Eight scans were examined to confirm the absence of artifacts or photoreduction and then averaged.
  • 24
    • 0343078890 scopus 로고    scopus 로고
    • note
    • 20b calibrated by fitting data for models of known structure.
  • 29
    • 0343514975 scopus 로고    scopus 로고
    • http://www.ncbi.nih.gov.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.