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Volumn 288, Issue 5469, 2000, Pages 1254-1256

Role for rapid dendritic protein synthesis in hippocampal mGluR- dependent long-term depression

Author keywords

[No Author keywords available]

Indexed keywords

METABOTROPIC RECEPTOR;

EID: 0034685813     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5469.1254     Document Type: Article
Times cited : (790)

References (28)
  • 11
    • 0343205324 scopus 로고    scopus 로고
    • note
    • 2. For recording, slices were placed in a submersion recording chamber, maintained at 30°C, and perfused with ACSF at a rate of 2 ml/min. FPs were evoked, recorded, and analyzed as described (27). Average values (±SEM) are expressed as the percentage of initial baseline response measured 55 to 60 min after DHPG or conditioning stimulation, unless otherwise indicated.
  • 12
  • 15
    • 0342770677 scopus 로고    scopus 로고
    • note
    • 7GpppG (n = 6). Both concentrations produced comparable inhibition of LTD relative to control cells (300 μM GTP; n = 5 and 6, respectively), so the data were combined. To ensure a reliable and robust LTD in all cases, we used 100 μM DHPG to induce LTD.
  • 18
    • 0343205322 scopus 로고    scopus 로고
    • note
    • For experiments on isolated CA1 dendrites, an incision was made at the border of the stratum pyramidale and stratum radiatum with a microdissection knife visualized under a dissection microscope (20). Slices were left to recover from the dendritic isolation procedure for at least 2 hours at 30°C. For recording, slices were placed in a submersion recording chamber, maintained at 30°C, and perfused with ACSF at a rate of 2 ml/min. Higher stimulation intensities (20 to 60 μA) were required to elicit FPs in isolated dendrites. Slices were used only if a slope of at least 0.1 mV/ms was obtained with a stimulation intensity of 100 μA. In these experiments, the absence of a population spike in response to high-intensity stimulation (150 μA) confirmed that the CA1 dendrites were isolated from their somata.
  • 24
    • 0031444409 scopus 로고    scopus 로고
    • K. C. Martin et al., Cell 91, 927 (1997).
    • (1997) Cell , vol.91 , pp. 927
    • Martin, K.C.1
  • 28
    • 0342335735 scopus 로고    scopus 로고
    • note
    • Special thanks to J. Fallon and J. Richter for providing essential advice, to W. C. Abraham for helpful discussions, and to P. Ornstein (Eli Lilly Company) for the gift of LY341495. Thanks to S. Meagher and E. Sklar for assistance. K.M.H. was supported by a grant from the National Eye Institute.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.