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Volumn 287, Issue 5456, 2000, Pages 1265-1269

Dopaminergic loss and inclusion body formation in α-synuclein mice: Implications for neurodegenerative disorders

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA SYNUCLEIN;

EID: 0034681471     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5456.1265     Document Type: Article
Times cited : (1621)

References (32)
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    • Mice were evaluated as described (19) with a rotorod (San Diego Instruments, San Diego, CA). Initially, they were trained for five trials. During the subsequent test trials, mice were placed individually on the cylinder and the speed of rotation increased from 0 to 40 rpm over a period of 240 s. The length of time mice remained on the rod (fall latency) was recorded and used as a measure of motor function.
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    • For Western blot analysis of TH levels, brains were homogenized and separated into cytosolic and particulate fractions as described (4). Twelve micrograms of cytosolic fraction per mouse were loaded onto 10% SDS-polyacrylamide gel etectrophoresis gels followed by transfer of proteins onto Immobilen membranes and detection of TH with a mAb to TH (1:1000; Roche). Blots were incubated with horseradish peroxidase-coupled secondary antibody and developed with the chemiluminescence reagent (NEN). After exposure of blots to film, the density of bands was quantitated with the ImageQuant system (Molecular Dynamics, Sunnyvale, CA).
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    • note
    • We thank S. Ordway and G. Howard for editorial assistance and M. Alford for technical assistance. Supported by NIH Grants AG5131 and AG10689 (to E.M.) and AG11385 (to LM.) and the Spencer Family Foundation (to M.H.).


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