Phage φ29 DNA polymerase residues involved in the proper stabilisation of the primer-terminus at the 3'-5' exonuclease active site

Journal of Molecular Biology

Volumn 304, Issue 1, 2000, Pages 1-9

  De Vega, Miguel ^a   Lázaro, José María ^a   Salas, Margarita ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

3' 5' exonuclease; N terminal domain; Site directed mutants; ssDNA binding; 29 DNA polymerase

Indexed keywords

DNA POLYMERASE; EXONUCLEASE; PRIMER DNA; SINGLE STRANDED DNA;

ARTICLE; BACILLUS PHAGE; CATALYSIS; CHEMICAL ANALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; DNA REPLICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME STABILITY; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOCOCCUS;

PROKARYOTA; THERMOCOCCUS; THERMOCOCCUS GORGONARIUS; UNIDENTIFIED BACTERIOPHAGE;

EID: 0034680587     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4178     Document Type: Article

References (40)

1. DNA polymerase β: Structure-function relationship from pre-steady kinetic analyses of all possible correct and incorrect base pairs for wild-type and R283A mutant
2. Fidelity of reverse transcriptase of human immunodeficiency virus type 2
3. Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: A two metal ion mechanism
4. Structure of DNA polymerase Klenow fragment bound to duplex DNA
5. A conserved 3'-5' exonuclease active site in prokaryotic and eukaryotic DNA polymerases
6. Characterization of a 3'-5' exonuclease activity in the phage φ29-encoded DNA polymerase
7. DNA polymerase insertion fidelity
8. Suggestions for 'safe' residue substitutions in site-directed mutagenesis
9. Compilation, alignment, and phylogenetic relationships of DNA polymerases
10. Enzymatic synthesis of deoxyribonucleic acid. XXXVI. A proofreading function for the 3'-5' exonuclease activity in deoxyribonucleic acid polymerases
11. A RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter
12. Prediction of the secondary structures of proteins from their amino acid sequence
13. Structure-function analysis of 3'-5' exonucleases of DNA polymerases
14. Primer-terminus stabilization at the catalytic active site of φ29 DNA polymerase. Involvement of two amino acid residues highly conserved in proofreading DNA polymerases
15. Mutational analysis of φ29 DNA polymerase residues acting as ssDNA ligands for 3'-5' exonucleolysis
16. φ29 DNA polymerase residue Ser122, a single-stranded DNA ligand for 3'-5' exonucleolysis, is required to interact with the terminal protein
17. Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage φ29 DNA polymerase
18. Kinetic partitioning between the exonuclease and polymerase sites in DNA error correction
19. Fidelity mechanisms in DNA replication
20. Fidelity of φ29 DNA polymerase. Comparison between protein-primed initiation and DNA polymerisation
21. 3'-5' exonuclease active site of φ29 DNA polymerase. Evidence favoring a metal ion-assisted reaction mechanism
22. Cocrystal structure of an editing complex of Klenow fragment with DNA
23. The bacteriophage φ29 DNA polymerase, a proof-reading enzyme
24. Analysis of the accuracy and implications of simple methods for predicting the secondary, structure of globular proteins
25. Crystal structure of a thermostable type B DNA polymerase from Thermococcus gorgonarius
26. Isolation and characterization of ten mutator alleles of the mitochondrial DNA polymerase-encoding MIP1 gene from Saccharomyces cerevisiae
27. Conformational coupling in DNA polymerase fidelity
28. Purification of φ29 DNA polymerase
29. 2GR
30. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP
31. Evidence that an archaeal alfa-like DNA polymerase has a modular organization of its associated catalytic activities
32. A separate editing exonuclease for DNA replication: The epsilon subunit of Escherichia coli DNA polymerase III holoenzyme
33. Base selection, proofreading and mismatch repair during DNA replication in Escherichia coli
34. Mutational analysis of the 3'-5' proofreading exonuclease of Escherichia coli DNA polymerase III
35. Fidelity of mammalian DNA replication and replicative DNA polymerases
36. φ29 DNA polymerase requires N-terminal domain to bind terminal protein and DNA primer substrates
37. 2-terminal fragment of T4 DNA polymerase and its complexes with single-stranded DNA and with divalent metal ions
38. Crystal structure of a pol α family replication DNA polymerase from bacteriophage RB69
39. DNA polymerase β: Pre-steady state kinetic analysis and roles of arginine-283 in catalysis and fidelity
40. An induced-fit mechanism for DNA replication fidelity: Direct measurement by single-turnover kinetics