Purification and DNA-binding properties of the Cro-type regulatory repressor protein Cng encoded by the Lactobacillus plantarum phage φg1e

Gene

Volumn 249, Issue 1-2, 2000, Pages 161-169

  Kakikawa, Makiko ^a   Ohkubo, Shingo ^a   Sakate, Toshihide ^a   Sayama, Michio ^a   Taketo, Akira ^b   Kodaira, Ken Ichi ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

^b UNIVERSITY OF FUKUI   (Japan)

Author keywords

DNA binding protein; Lactobacillus phage; Operator; Promoter; Repressor

Indexed keywords

DNA BINDING PROTEIN; DNA FRAGMENT; PROTEIN; REPRESSOR PROTEIN;

ARTICLE; BACTERIOPHAGE; DNA BINDING; DNA FOOTPRINTING; DNA SUPERCOILING; ESCHERICHIA COLI; LACTOBACILLUS PLANTARUM; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERATOR GENE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CROSS LINKING;

BACTERIOPHAGES; BASE SEQUENCE; BINDING SITES; CROSS-LINKING REAGENTS; DEOXYRIBONUCLEASE I; DIMERIZATION; DNA FOOTPRINTING; DNA, VIRAL; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; REPRESSOR PROTEINS; VIRAL NONSTRUCTURAL PROTEINS;

ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS PLANTARUM;

EID: 0034673922     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00146-3     Document Type: Article

References (15)

1. Aki T., Adhya S. Repressor induced site-specific binding of HU for transcriptional regulation. EMBO J. 16:1997;3666-3674.
2. Boyce J.D., Davidson B.E., Hillier A.J. Identification of prophage genes expressed in lysogenes of the Lactococcus lactis bacteriophage BK5-T. Appl. Environ. Microviol. 61:1995;4099-4104.
3. Campbell A. Comparative molecular biology of lambdoid phages. Annu. Rev. Microbiol. 48:1994;193-222.
4. Dodd I.B., Egan J.B. DNA binding by the coliphage 186 repressor protein CI. J. Biol. Chem. 271:1996;11 532-11 540.
5. Engel G., Altermann E., Klein J.R., Henrich B. Structure of a genome region of the Lactobacillus gasseri temperate phage φadh covering a repressor gene and cognate promoters. Gene. 210:1998;61-70.
6. Garcia P., Ladero V., Alonso J.C., Suarez J.E. Cooperative interaction of CI protein regulates lysogeny of Lactobacillus casei by bacteriophage A2. J. Virol. 73:1999;3920-3929.
7. Kakikawa M., Watanabe N., Funawatashi T., Oki M., Yasukawa H., Taketo A., Kodaira K.-I. Promoter/repressor system of Lactobacillus plantarum phage φg1e: characterization of the promoters pR49-pR-pL and overproduction of the cro-like protein Cng in Escherichia coli. Gene. 215:1998;371-379.
8. L, the repressor gene cpg, and the cpg-encoded protein Cpg in Escherichia coli. Gene. 242:2000;155-166.
9. Kodaira K.-I., Oki M., Kakikawa M., Watanabe N., Hirakawa M., Yamada K., Taketo A. Genome structure of the Lactobacillus temperate phage φg1e: the whole genome sequence and the putative promoter/repressor system. Gene. 187:1997;45-53.
10. Lamont I., Brumby A.M., Egan J.B. UV induction of coliphage 186: prophage induction as an SOS function. Proc. Natl. Acad. Sci. USA. 86:1989;5492-5496.
11. McDonnell G.E., McConnell D.J. Overproduction isolation and DNA-binding characteristics of Xre the repressor protein from the Bacillus subtilis defective prophage PBSX. J. Bacteriol. 176:1994;5831-5834.
12. Nauta A., van Sinderen D., Karsens H., Smit E., Venema G., Kok J. Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t. Mol. Microbiol. 19:1996;1331-1341.
13. Neufing P.J., Shearwin K.E., Camerotto J., Egan J.B. The CII protein of bacteriophage 186 establishes lysogeny by activating a promoter upstream of the lysogenic promoter. Mol. Microbiol. 21:1996;751-761.
14. Oki M., Kakikawa M., Nakamura S., Yamamura E.T., Watanabe K., Sasamoto M., Taketo A., Kodaira K.-I. Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage φg1e: analysis in Escherichia coli system. Gene. 197:1997;137-145.
15. Van de Guchte M., Daly C., Fizgerald G.F., Arendt E.K. Identification of the putative repressor-encoding gene cI of the temperate lactococcal bacteriophage Tuc2009. Gene. 144:1994;93-95.