Rapid gene inactivation in Pseudomonas aeruginosa

FEMS Microbiology Letters

Volumn 193, Issue 2, 2000, Pages 201-205

  Windgassen, Merle ^a   Urban, Andreas ^a   Jaeger, Karl Erich ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Insertional mutagenesis; Polar effect; Pseudomonas aeruginosa

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE INACTIVATION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

CONJUGATION, GENETIC; ESCHERICHIA COLI; GENE TARGETING; GENES, BACTERIAL; GENETIC VECTORS; LIPASE; MUTAGENESIS, INSERTIONAL; OPERON; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0034672395     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00476-6     Document Type: Article

References (21)

1. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
2. The pKNOCK series of broad host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of Gram-negative bacteria
3. Use of targeted insertional mutagenesis to determine whether chondroitin lyase II is essential for chondroitin sulfate utilization by Bacteroides thetaiotaomicron
4. A rapid method for disrupting genes in the Escherichia coli genome
5. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
6. Constructs for insertional mutagenesis, transcriptional signal localization and gene regulation studies in root nodule and other bacteria
7. A vector for systematic gene inactivation in Bacillus subtilis
8. Pseudomonas aeruginosa LasB mutant constructed by insertional mutagenesis reveals elastolytic activity due to alkaline proteinase and the LasA fragment
9. XcmI-containing vector for direct cloning of PCR products
10. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis
11. - bacteria
12. RNA processing modulates the expression of the arcDABC operon in Pseudomonas aeruginosa
13. A broad host range mobilization system for in vivo genetic engineering: Transposong mutagenesis in Gram-negative bacteria
14. Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: Cloning of a gene, encoding one of the esterases
15. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens
16. Chromosomal genetics of Pseudomonas
17. Bacterial lipases
18. Studies on transformation of Escherichia coli with plasmids
19. pBBR1MCS: A broad-host-range cloning vector