Kinetic alteration of a human dihydrodiol/3α-hydroxysteroid dehydrogenase isoenzyme, AKR1C4, by replacement of histidine-216 with tyrosine or phenylalanine

Biochemical Journal

Volumn 352, Issue 3, 2000, Pages 685-691

  Ohta, T ^a   Ishikura, S ^a   Shintani, S ^a   Usami, N ^a   Hara, A ^a  

^a GIFU PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

Aldo keto reductase; Coenzyme binding; Non essential activator; Substrate binding cleft

Indexed keywords

BILE ACID; BROMSULFOPHTHALEIN; CLOFIBRIC ACID; DIHYDRODIOL 3ALPHA HYDROXYSTEROID DEHYDROGENASE; DIHYDRODIOL DEHYDROGENASE; ESTRADIOL; FLUFENAMIC ACID; HEXESTROL DERIVATIVE; HISTIDINE; HYDROXYSTEROID DEHYDROGENASE; ISOENZYME; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PHENOLPHTHALEIN; PHENYLALANINE; THYROXINE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL MUTAGENESIS; CHEMICAL REACTION KINETICS; COENZYME; COMPETITIVE INHIBITION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

3-HYDROXYSTEROID DEHYDROGENASES; AMINO ACID SUBSTITUTION; BILE ACIDS AND SALTS; BINDING SITES; BINDING, COMPETITIVE; COENZYMES; ENZYME ACTIVATION; HISTIDINE; HUMANS; ISOENZYMES; KINETICS; MUTATION; PHENYLALANINE; THERMODYNAMICS; TYROSINE;

EID: 0034672220     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3520685     Document Type: Article

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