Microwave pretreatment of sections to improve the immunocytochemical detection of progesterone receptors in the guinea pig hypothalamus

Journal of Neuroscience Methods

Volumn 104, Issue 1, 2000, Pages 27-34

  Warembourg, M ^a   Leroy, D ^a  

^a INSERM   (France)

Author keywords

Brain; Immunohistochemistry; Microwave irradiation; Paraffin embedded tissue; Progesterone receptor

Indexed keywords

PROGESTERONE RECEPTOR;

ANIMAL TISSUE; ARTICLE; FEMALE; FROZEN SECTION; GUINEA PIG; HYPOTHALAMUS; IMMUNOCYTOCHEMISTRY; MICROWAVE IRRADIATION; NONHUMAN; PH; PRIORITY JOURNAL; TECHNIQUE;

ANIMALS; BUFFERS; CITRATES; FEMALE; GUINEA PIGS; HYDROGEN-ION CONCENTRATION; HYPOTHALAMUS; IMMUNOHISTOCHEMISTRY; MICROTOMY; MICROWAVES; NEURONS; RECEPTORS, PROGESTERONE;

EID: 0034671154     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00320-4     Document Type: Article

References (24)

1. New monoclonal antibodies to estrogen and progesterone receptors effective for paraffin section immunohistochemistry
2. Immunocytochemical localization of estrogen-induced progestin receptors in guinea pig
3. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections
4. Estrogen plus progesterone increases progestin receptor immunoreactivity in the brain of ovariectomized guinea pigs
5. Microwave-stimulated antigen retrieval is pH and temperature dependent
6. Effects of microwave pretreatment on immunocytochemical staining of vibratome sections and tissue blocks of human cerebral cortex stored in formaldehyde fixative for long periods
7. An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative
8. Androgen receptor immunostaining and its tissue distribution in formalin-fixed, paraffin-embedded sections after microwave treatment
9. Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment
10. A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections
11. Immunocytochemical localization of androgen receptor with polyclonal antibody in paraffin-embedded human tissues
12. Monoclonal antibodies to rabbit progesterone receptor: crossreaction with other mammalian progesterone receptors
13. One-step immunoaffinity purification of active progesterone receptor. Further evidence in favor of the existence of a single steroid binding subunit
14. The rabbit progesterone receptor. Evidence for a single steroid-binding subunit and characterization of receptor mRNA
15. A rapid method of epitope mapping. Application to the study of immunogenic domains and to the characterization of various forms of rabbit progesterone receptor
16. Microwave-cryostat technique for neuroanatomical studies
17. Immunocytochemical detection of androgen receptor in human temporal cortex: characterization and application of polyclonal androgen receptor antibodies in frozen and paraffin-embedded tissues
18. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
19. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry
20. Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections
21. Microwave stabilization enhances immunocytochemical detection of estrogen receptor in frozen sections of macaque oviduct
22. Immunocytochemical localization of progesterone receptor in the guinea pig central nervous system
23. Immunohistochemical evidence of the presence of estrogen and progesterone receptors in the same neurons of the guinea pig hypothalamus and preoptic area