NMR structures of thioredoxin m from the green alga Chlamydomonas reinhardtii

Proteins: Structure, Function and Genetics

Volumn 41, Issue 3, 2000, Pages 334-349

  Lancelin, Jean Marc ^a,d   Guilhaudis, Laure ^b   Krimm, Isabelle ^a   Blackledge, Martin J ^b   Marion, Dominique ^b   Jacquot, Jean Pierre ^c  

^a UNIVERSITÉ LYON 1   (France)

^b CEA GRENOBLE   (France)

^c UNIVERSITÉ DE LORRAINE   (France)

^d Laboratoire de RMN Biomoleculaire   (France)

Author keywords

Chloroplast thioredoxin; Cysteine reactivity; Dithiol redox control; Enzymatic photoregulation; NADP malate dehydrogenase

Indexed keywords

THIOREDOXIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHLAMYDOMONAS REINHARDTII; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

ALGAE; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; EUKARYOTA; PROKARYOTA;

EID: 0034669781     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0134(20001115)41:3<334::AID-PROT60>3.0.CO;2-M     Document Type: Article

References (81)

1. Thioredoxins: Structure and function in plant cells New Phytol
2. In vivo functional discrimination between plant thioredoxins by heterologous expression in the yeast Saccharomyces cerevisiae
3. Molecular aspects of components of the ferredoxin/thioredoxin systems in Chlamydomonas reinhardtii
4. Structure of oxidized thioredoxin to 4 with 5 Å resolution
5. Three-dimensional structure of Escherichia coli thioredoxin to 2.8 Å resolution
6. Crystal structure of thioredoxin from Escherichia coli at at 1.68 Å resolution
7. High-resolution solution structures of oxidized and reduced Escherichia coli thioredoxin
8. Evidence for five divergent thioredoxin h sequences in Arabidopsis thaliana
9. In vivo characterization of a thioredoxin h target protein defines a new peroxiredoxin family
10. Identification and functional characterization of a novel mitochondrial thioredoxin system in Saccharomyces cerevisiae
11. Purification and characterization of a second type thioredoxin peroxidase type II TPx from Saccharomyces cerevisiae
12. A new antioxidant with alkyl hydroperoxide defense properties in yeast
13. An internal cysteine is involved in the thioredoxin-dependent activation of sorghum leaf NADP-malate dehydrogenase
14. The autoinhibition of sorghum NADP malate dehydrogenase is mediated by a C-terminal negative charge
15. Structural basis for light activation of a chloroplast enzyme: The structure of Sorghum NADP-malate dehydrogenase in its oxidized form
16. Chloroplast NADP-malate dehydrogenase: Structural basis of light-dependent regulation of activity by thiol oxidation and reduction
17. Light-dependent activation of NADP-malate dehydrogenase: A complex process
18. Primary structure of sorghum malate dehydrogenase NADP deduced from cDNA sequence Homology with malate dehydrogenase NAD
19. Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase
20. Site-directed mutagenesis reveals the involvement of an additional thioredoxin-dependent regulatory site in the activation of recombinant sorghum leaf NADP-malate dehydrogenase
21. Identification and characterization of the second regulatory disulfide bridge of recombinant sorghum leaf NADP-malate dehydrogenase
22. Determination of the regulatory disulfide bonds of NADP-dependent malate dehydrogenase from Pisum sativum by site-directed mutagenesis
23. Direct NMR observation of the thioredoxin-mediated reduction of the chloroplast NADP-malate dehydrogenase provides a structural basis for the relief of autoinhibition
24. Studies on the mechanism of the reductive activation of NADP-malate dehydrogenase by thioredoxin m and low-molecular-weight thiols
25. 13C NMR
26. 1H NMR
27. Chlamydomonas reinhardtii thioredoxins: Structure of the genes coding for the chloroplastic m and cytosolic h isoforms; expression in Escherichia coli of the recombinant proteins purification and biochemical properties
28. GIFA V4: A complete package for NMR data set processing
29. NMRPipe: A multidimensional spectral processing system based on UNIX pipes
30. NMR solution stuctures of an oxidised thioredoxin h from the eukaryotic green alga Chlamydomonas reinhardtii
31. A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients
32. 1H spin system identification in macromolecules
33. 15N NMR relaxation
34. Rapid recording of 2D NMR spectra without phase cycling: Application to the study of hydrogen exchange in proteins
35. Solution structure rotational diffusion anisotropy and local backbone dynamics of Rhodobacter capsulatus cytochrome c2
36. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. Analysis of experimental results
37. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. Theory and range of validity
38. 1H NMR spectroscopy
39. Backbone dynamics of Escherichia coli ribonuclease HI: Correlations with structure and function in an active enzyme
40. 15N resonance assignments of oxidized thioredoxin h from the eukaryotic green alga Chlamydomonas reinhardtii using new methods on two-dimensional triple-resonance NMR spectroscopy and computer-assisted backbone assignment
41. Structure and dynamics of ferrocytochrome c553 from Desulfovibrio vulgaris studied by NMR spectroscopy and restrained molecular dynamics
42. Determination of three-dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms circumventing problems associated with folding
43. Molecular dynamics simulations with experimental restraints
44. MOLMOL: A program for display and analysis of macromolecular structures
45. AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR
46. The anatomy and taxonomy of protein structure
47. Mapping the protein universe
48. Dictionary of recurrent domains in protein structures
49. Touring protein fold space with Dali/FSSP
50. The Protein Data Bank: A computer-based archival file for macromolecular structures
51. Crystal structure of thioredoxin-2 from Anabaena
52. The high-resolution three-dimensional solution structures of the oxidized and reduced states of human thioredoxin
53. Crystal structures of reduced oxidized and mutated human thioredoxins: Evidence for a regulatory homodimer
54. General acid/base in the active site of Escherichia coli thioredoxin
55. Human thioredoxin homodimers: Regulation by pH role of aspartate 60 and crystal structure of the aspartate 60 → asparagine mutant
56. Biochemical characterization of thioredoxin 1 from Dictyostelium discoideum
57. 15N resonance assignments and secondary structure of the human thioredoxin C62A C69A C73A mutant
58. Is human thioredoxin monomeric or dimeric?
59. 15N NMR relaxation measurements
60. The conserved buried aspartic acid in oxidized Escherichia coli thioredoxin has a pK(a) of 7.5. Its titration produces a related shift in global stability
61. 1H NMR
62. 13C NMR
63. Ionization equilibria for side-chain carboxyl groups in oxidized and reduced human thioredoxin and in the complex with its target peptide from the transcription factor NFκ B
64. Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli
65. Determination of the pK(a) values of active-center cysteines: Cysteines 32 et 35 in Escherichia coli thioredoxin by Raman spectroscopy
66. Aspartic acid 26 in reduced Escherichia coli thioredoxin has a pK(a) > 9
67. Proton sharing between cysteine thiols in Escherichia coli thioredoxin: Implications for the mechanism of protein disulfide reduction
68. Microscopic pK(a) values of Escherichia coli thioredoxin
69. Rates of thiol-disulfide interchange reactions between mono- and dithiols and Ellman's reagents
70. Ionisation of cysteine residues at the termini of model alpha-helical peptides Relevance to unusual thiol pK(a) values in proteins of the thioredoxin family
71. Substitution of the conserved tryptophan 31 in Escherichia coli thioredoxin by site-direct mutagenesis and structure-function analysis
72. Characterization of Escherichia coli thioredoxins with altered active site residues
73. Mimicking the active site of protein disulfide-isomerase by substitution of proline 34 in Escherichia coli thioredoxin
74. The CXXC motif: Imperatives for the formation of native disulfides bonds in the cell
75. The CXXC motif: A rheostat in the active site