Exocytosis requires asymmetry in the central layer of the SNARE complex

EMBO Journal

Volumn 19, Issue 22, 2000, Pages 6000-6010

  Ossig, Rainer ^a,b   Schmitt, Hans Dieter ^a   De Groot, Bert ^a   Riedel, Dietmar ^a   Keränen, Sirkka ^c   Ronne, Hans ^d   Grubmüller, Helmut ^a   Jahn, Reinhard ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^d SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Exocytosis; Membrane fusion; SNARE; Yeast

Indexed keywords

ARGININE; GLUTAMINE; LEUCINE ZIPPER PROTEIN; SNARE PROTEIN;

ALLELE; ARTICLE; CRYSTAL STRUCTURE; EUKARYOTE; EXOCYTOSIS; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN SECONDARY STRUCTURE; YEAST CELL;

EUKARYOTA;

EID: 0034669052     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.22.6000     Document Type: Article

References (58)

1. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane-proteins that function in vesicular transport
2. Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast
3. Interaction models for water in relation to protein hydration
4. Molecular dynamics with coupling to an external bath
5. Avoiding singularities and numerical instabilities in free-energy calculations based on molecular simulations
6. Kex2-dependent invertase secretion as a tool to study the targeting of transmembrane proteins which are involved in ER-Golgi transport in yeast
7. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis
8. 2+ and drives membrane fusion
9. Yeast Snc proteins complex with Sec9. Functional interactions between putative SNARE proteins
10. Involvement of long-chain fatty-acid elongation in the trafficking of secretory vesicles in yeast
11. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
12. Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs
13. Mixed and non-cognate SNARE complexes - Characterization of assembly and biophysical properties
14. Folding intermediates of SNARE complex assembly
15. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways
16. Conserved α-helical segments on yeast homologs of the synaptobrevin/VAMP family of v-SNAREs mediate exocytic function
17. A new beat for the SNARE drum
18. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy
19. Membrane fusion and exocytosis
20. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway
21. Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex
22. Structural rearrangements of tubulin and actin during the cell cycle of Saccharomyces
23. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
24. Structural organization of the synaptic exocytosis core complex
25. Improvements in epoxy resin embedding methods
26. Intracellular membrane fusion: SNAREs only?
27. Raster3D: Photorealistic molecular graphics
28. Homotypic vacuolar fusion mediated by t- and v-SNAREs
29. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway
30. Organelle membrane-fusion - A novel function for the syntaxin homolog Ufe1p in ER membrane-fusion
31. Transport-vesicle targeting: Tethers before SNAREs
32. Homologs of the synaptobrevin vamp family of synaptic vesicle proteins function on the late secretory pathway in Saccharomyces cerevisiae
33. Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro
34. Studies on free energy calculations. I. Thermodynamic integration using a polynomial path
35. Formation of a yeast SNARE complex is accompanied by significant structural changes
36. Mechanism of intracellular protein transport
37. Numerical integration of the cartesian equations of motion of a system with constraints; molecular dynamics of n-alkanes
38. The synaptobrevin-related domains of Bos1p and Sec22p bind to the syntaxin-like region of Sed5p
39. A ras-like protein is required for a post-Golgi event in yeast secretion
40. A system of yeast shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
41. SNAP receptors implicated in vesicle targeting and fusion
42. Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole
43. A yeast GTPase-activating protein that interacts specifically with a member of the Ypt/ Rab family
44. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
45. Phylogenetic analysis of membrane trafficking proteins: A family reunion and secondary structure predictions
46. Defining the functions of trans-SNARE pairs
47. Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion
48. A molecular dynamics study of the decane/water interface
49. SNAREpins - Minimal machinery for membrane-fusion
50. Exocytotic mechanism studied by truncated and zero layer mutants of the C-terminus of SNAP-25
51. A conserved domain is present in different families of vesicular fusion proteins - A new superfamily
52. A model for structural similarity between different SNARE complexes based on sequence relationships
53. SNARE interactions are not selective - Implications for membrane fusion specificity