In situ assay for identifying inhibitors of bacterial transglycosylase

FEMS Microbiology Letters

Volumn 191, Issue 2, 2000, Pages 187-190

  Branstrom, Arthur A ^a   Midha, Sunita ^a   Goldman, Robert C ^a  

^a Advanced Medicine East   (United States)

Author keywords

Cell wall; Lipid II; Moenomycin; Peptidoglycan; Transglycosylase

Indexed keywords

AMPICILLIN; BACITRACIN; BAMBERMYCIN; GLYCOSYLTRANSFERASE; LIPID II; PEPTIDOGLYCAN; TRITON X 100; TUNICAMYCIN; UNCLASSIFIED DRUG;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL CELL WALL; CONTROLLED STUDY; DRUG DEVELOPMENT; ENZYME GLYCOSYLATION; ENZYME INHIBITION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; RADIOASSAY;

ANTIBIOTICS; BACTERIOLOGICAL TECHNIQUES; BAMBERMYCINS; ENZYME INHIBITORS; ESCHERICHIA COLI; GLYCOSYLTRANSFERASES; OCTOXYNOL; PEPTIDOGLYCAN; POLYISOPRENYL PHOSPHATE OLIGOSACCHARIDES; TUNICAMYCIN;

EID: 0034668289     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00388-8     Document Type: Article

References (11)

1. van Heijenoort Y., Leduc M., Singer H., van Heijenoort J. Effects of moenomycin on Escherichia coli. J. Gen. Microbiol. 133:1987;667-674.
2. van Heijenoort Y., Gomez M., Derrien M., Ayala J., van Heijenoort J. Membrane intermediates in the peptidoglycan metabolism of Escherichia coli: possible roles of PBP1b and PBP3. J. Bacteriol. 174:1992;3549-3557.
3. Branstrom, A.A., Midha, S., Longley, C.B., Han, K., Baizman, E.R. and Axelrod, H.R. (1998) Development of an assay for identifying inhibitors specific for MraY translocase and MurG transferase, p. 100, C108. 38th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, CA.
4. Branstrom A.A., Midha S., Longley C.B., Han K., Baizman E.R., Axelrod H.R. Assay for identification of inhibitors for bacterial MraY translocase or MurG transferase. Anal. Biochem. 280:2000;315-319.
5. Ikeda M., Wachi M., Jung H.K., Ishimo F., Matsuhashi M. The Escherichia coli mraY gene encoding UDP-N-acetyl-muramyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase. J. Bacteriol. 173:1991;1021-1026.
6. Mengin-Lecreulx D., Texier L., Rousseau M., van Heijenoort J. The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis. J. Bacteriol. 173:1991;4625-4636.
7. Kohlrausch U., Holtje J.V. One-step purification procedure for UDP-N-acetylmuramyl-peptide murein precursors from Bacillus cereus. FEMS Microbiol. Lett. 62:1991;253-257.
8. Anderson J.S., Meadow P.M., Haskin M.A., Strominger J.L. Biosynthesis of the peptidoglycan of bacterial cell walls. I. Utilization of uridine diphosphate acetylmuramyl pentapeptide and uridine diphosphate acetylglucosamine for peptidoglycan synthesis by particulate enzymes from Staphylococcus aureus and Micrococcus lysodeikticus. Arch. Biochem. Biophys. 116:1966;487-515.
9. Lo M.-C., Men H., Branstrom A., Helm J., Yao N., Goldman R., Walker S. A new mechanism of action proposed for ramoplanin. J. Am. Chem. Soc. 122:2000;3540-3541.
10. Goldman R.C., Baizman E.R., Longley C.B., Branstrom A.A. Chlorobiphenyl-desleucyl-vancomycin inhibits the transglycosylation process required for peptidoglycan synthesis in bacteria in the absence of dipeptide binding. FEMS Microbiol. Lett. 183:2000;209-214.
11. van Heijenoort Y., Derrien M., van Hiejenoort J. Polymerization by transglycosylation in the biosynthesis of the peptidoglycan of Escherichia coli K12 and its inhibition by antibiotics. FEBS Lett. 89:1978;141-144.