Amount of paraformaldehyde for sample fixation markedly affects the quality of platelet assessment by flow cytometry-experience from two clinical trials

Thrombosis Research

Volumn 99, Issue 6, 2000, Pages 643-644

  Fuzaylov, Sergey Y ^a   Serebruany, Victor L ^a  

^a SINAI HOSPITAL OF BALTIMORE   (United States)

Author keywords

Clinical trials; Fixation; Flow cytometry; Platelets

Indexed keywords

FIXATIVE; PARAFORMALDEHYDE;

BLOOD ANALYSIS; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; LETTER; PRIORITY JOURNAL; QUALITY CONTROL; SAMPLE; THROMBOCYTE; TISSUE FIXATION;

BLOOD PLATELETS; CLINICAL TRIALS; CORONARY DISEASE; DOSE-RESPONSE RELATIONSHIP, DRUG; FIXATIVES; FLOW CYTOMETRY; FORMALDEHYDE; HUMANS; POLYMERS;

EID: 0034666726     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0049-3848(00)00285-1     Document Type: Letter

References (3)

1. Allain J.P., Cooper H.A., Wagner R.H., Brinkhous K.M. Platelets fixed with paraformaldehyde. a new reagent for assay of von Willebrand factor and platelet aggregating factor J Lab Clin Med. 85:1975;318-328.
2. Matic G.B., Chapman E.S., Zaiss M., Rothe G., Schmitz G. Whole blood analysis of reticulated platelets. improvements of detection and assay stability Cytometry. 34:1998;229-234.
3. Kuhne T., Hornstein A., Semple J., Chang W., Blanchette V., Freedman J. Flow cytometric evaluation of platelet activation in blood collected into EDTA vs. Diatube-H, a sodium citrate solution supplemented with theophylline, adenosine, and dipyridamole. Am J Hematol. 50:1995;40-45.