Hydrophobic character of surface regions and total hydrophobicity of four variants of chromosomal class C β-lactamase from Pseudomonas aeruginosa are identical. Chromatographic comparison of the hydrophobic character of the variants and the effect of focusing buffer composition on the separation of the variants by chromatofocusing with internal and external pH gradients

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 746, Issue 2, 2000, Pages 161-172

  Walther Rasmussen, Jan ^a   Høiby, Niels ^a  

^a Rigshospitalet   (Denmark)

Author keywords

Chromatofocusing; Enzymes; pH gradient; Lactamase

Indexed keywords

BETA LACTAMASE;

ANION EXCHANGE; ARTICLE; CHROMATOFOCUSING; CHROMATOGRAPHY; HYDROPHOBICITY; ISOELECTRIC FOCUSING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PSEUDOMONAS AERUGINOSA; REVERSED PHASE LIQUID CHROMATOGRAPHY; SURFACE CHARGE;

BETA-LACTAMASES; BUFFERS; CHROMATOGRAPHY, LIQUID; CHROMOSOMES, BACTERIAL; HYDROGEN-ION CONCENTRATION; PSEUDOMONAS AERUGINOSA; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0034666506     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(00)00315-7     Document Type: Article

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