Inhibition of a ribosome-inactivating ribonuclease: The crystal structure of the cytotoxic domain of colicin E3 in complex with its immunity protein

Structure

Volumn 8, Issue 9, 2000, Pages 949-960

  Carr, Stephen ^a   Walker, Daniel ^a   James, Richard ^a   Kleanthous, Colin ^a   Hemmings, Andrew M ^a  

^a UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

Crystal structure; Immunity protein; Ribonuclease colicin; Ribosome inactivation

Indexed keywords

COLICIN; COLICIN E3; DEOXYRIBONUCLEASE; IMMUNITY PROTEIN; INHIBITOR PROTEIN; RIBONUCLEASE; RIBOSOME INACTIVATING PROTEIN; RNA 16S; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; RNA CLEAVAGE; STRUCTURE ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034665456     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00186-6     Document Type: Article

References (57)

1. The biology of E colicins: Paradigms and paradoxes
2. Structure and dynamics of the colicin E1 channel Mol
3. Colicin E8, a DNase which indicates an evolutionary relationship between colicin E2 and colicin E3
4. Complete nucleotide sequence of the colicin E9 (cei) gene
5. Cloning and characterisation of the cole7 plasmid
6. A cytotoxic ribonuclease targeting specific transfer RNA anticodons
7. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli
8. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo
9. Purification and Characterisation of active component and active fragment of Colicin E3
10. Inactivation of ribosomes in vitro by Colicin E3
11. X-ray structure of 70S ribosome functional complexes
12. Recognition of the codon-anticodon helix by ribosomal RNA
13. Identification of a unique specificity determinant of the colicin E3 immunity protein
14. Crystal structure of colicin E3 immunity protein: An inhibitor of a ribosome inactivating RNase
15. The three dimensional structure of the colicin-E3 immunity protein by distance geometry calculation
16. Immunity proteins and their specificity for endonuclease colicins: Right from wrong in protein-protein recognition
17. Protein-protein interactions in colicin E9 DNase - Immunity protein complexes. 1. Diffusion controlled association and femtomolar binding for the cognate complex
18. Purification and properties of colicin E3 immunity protein
19. Specificity in protein-protein recognition: Conserved Im9 residues are the major determinants of stability in the colicin E9 DNase-Im9 complex
20. Dual recognition and the role of specificity-determining residues in colicin E9 DNase-immunity protein interactions
21. The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein
22. Structural and mechanistic basis of immunity towards endonuclease colicins
23. Structural studies on Colicin E3 and its immunity protein
24. Crystallisation and preliminary crystallographic analysis of colicin E3 RNase domain in complex with its cognate immunity protein
25. The third IgG-binding domain from streptococcal protein G. An analysis by X-ray crystallography of the structure alone and in a complex with Fab
26. Filamentous phage infection: Crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA
27. The ribonuclease activity of the cytotoxin α-sarcin. The characteristics of the enzymatic activity of α-sarcin with ribosomes and ribonucleic acids as substrates
28. Protein structure comparison by alignment of distance matrices
29. A WWW service system for automatic comparison of protein structures
30. Protein-protein recognition: Crystal structural analysis of a Barnase-Barstar complex at 2.0 Å resolution
31. The atomic structure of protein-protein recognition sites
32. Shape complimentarity at protein-protein interfaces
33. SURFNET - A program for visualising molecular-surfaces, cavities and intermolecular interactions
34. Principles of protein-protein interaction
35. Surface, subunit interfaces and interior of oligomeric proteins
36. Molecular evolution of E colicin plasmids with emphasis on the endonuclease colicins
37. Arginine-mediated recognition: Arginine fork
38. DNA sequence analysis of three missense mutations affecting colicin E3 bactericidal activity
39. Crystal structures of ribonuclease A complexes with 5' diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 Å resolution
40. Molecular structure of a new family of ribonucleases
41. Subsite binding in an RNase: Structure of a Barnase-tetranucleotide complex at 1.76 Å resolution
42. Conformational flexibility in single-stranded oligonucleotides: Crystal structure of a hydrated calcium salt of adenylyl-(3'-5')-adenosine
43. Processing of X-ray diffraction data collected in oscillation mode
44. Data reduction
45. Automated structure solution for MIR and MAD
46. Joint CCP4 and ESF-EACBM newsletter on protein crystallography
47. Improved methods for building protein models in electron-density maps and the location of errors in these models
48. Free R value - A novel statistical quantity for assessing the accuracy of crystal structures
49. Crystallography and NMR system: A new software suite for macromolecular structure determination
50. AMoRe: An automated molecular replacement program package
51. Databases in protein crystallography
52. PROCHECK - A program to check the stereochemical quality of protein structures
53. The CCP4 suite - Programs for protein crystallography
54. MOLSCRIPT - A program to produce detailed and schematic plots of protein structures
55. Raster3D: Photorealistic molecular graphics