Targeting and insertion of C-terminally anchored proteins to the mitochondrial outer membrane is specific and saturable but does not strictly require ATP or molecular chaperones

Biochemical Journal

Volumn 349, Issue 2, 2000, Pages 611-621

  Lan, Ling ^a   Isenmann, Sandra ^a   Wattenberg, Binks W ^a  

^a INSTITUTE OF MEDICAL AND VETERINARY SCIENCE   (Australia)

Author keywords

Import pathway; Protein import; Rat liver mitochondria; Tail anchored membrane protein; VAMP 1

Indexed keywords

ADENOSINE TRIPHOSPHATE; CHAPERONE; CYTOPLASM PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MEMBRANE PROTEIN; PHOSPHORUS 32; SYNAPTOBREVIN;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; MEMBRANE VESICLE; MITOCHONDRIAL MEMBRANE; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN TARGETING; RAT;

ADENOSINE TRIPHOSPHATE; ANIMALS; BIOLOGICAL TRANSPORT; CELL-FREE SYSTEM; CYTOSOL; INTRACELLULAR MEMBRANES; MEMBRANE PROTEINS; MITOCHONDRIA, LIVER; MOLECULAR CHAPERONES; PROTEIN ISOFORMS; PROTEIN STRUCTURE, TERTIARY; R-SNARE PROTEINS; RATS;

EID: 0034661510     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3490611     Document Type: Article

References (32)

1. 1 Kutay, U., Hartmann, E. and Rapoport, T. A. (1993) A class of membrane proteins with a C-terminal anchor. Trends Cell Biol. 3, 72-75
2. 5 to phospholipid vesicles and biological membranes. Effect of orientation on intermembrane transfer and digestion by carboxypeptidase Y. J. Biol. Chem. 254, 6483-6488
3. 5. Proc. Natl. Acad. Sci. U.S.A. 80, 7249-7253
4. 4 Kim, P. K., Janiak-Spens, F., Trimble, W. S., Leber, B. and Andrews, D. W. (1997) Evidence for multiple mechanisms for membrane binding and integration via carboxyl-terminal insertion sequences. Biochemistry 36, 8873-8882
5. 5 suggests novel targeting pathways. J. Biol. Chem. 268, 2802-2808
6. 6 Krajewski, S., Tanaka, S., Takayama, S., Schibler, M. J., Fenton, W. and Reed, J. C. (1993) Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res 53, 4701-4714
7. 7 Lithgow, T., van Driel, R., Bertram, J. F. and Strasser, A. (1994) The protein product of the oncogene bcl-2 is a component of the nuclear envelope, the endoplasmic reticulum, and the outer mitochondrial membrane. Cell Growth Differ. 5, 411-417
8. 8 Kutay, U., Ahnert-Hilger, G., Hartmann, E., Wiedenmann, B. and Rapoport, T. A. (1995) Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane. EMBO J. 14, 217-223
9. 9 Millar, D. G. and Shore, G. C. (1996) Signal anchor sequence insertion into the outer mitochondrial membrane. J. Biol. Chem. 271, 25823-25829
10. 10 Kim, P. K., Hollerbach, C., Trimble, W. S., Leber, B. and Andrews, D. W. (1999) Identification of the endoplasmic reticulum signal in vesicle associated membrane proteins. J. Biol. Chem. 274, 36876-36882
11. 11 Isenmann, S., Khew-Goodall, Y, Gamble, J., Vadas, M. and Wattenberg, B. W. (1998) A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal. Mol Biol. Cell 9, 1649-1660
12. 5. J. Biol. Chem. 273, 31097-31102
13. 13 Neupert, W., Hartl, F. U., Craig, E. A. and Pfanner, N. (1990) How do polypeptides cross the mitochondrial membranes? Cell 63, 447-450
14. 14 Pfanner, N., Craig, E. A. and Honlinger, A. (1997) Mitochondrial preprotein translocase. Annu. Rev. Cell Dev. Biol. 13, 25-51
15. 15 Hachiya, N., Alam, R., Sakasegawa, Y., Sakaguchi, M., Mihara, K. and Omura, T. (1993) A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins. EMBO J 12, 1579-1586
16. 16 Deshaies, R. J., Koch B. D., Werner-Washburne, M., Craig E. A. and Schekman, R. (1988) A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature (London) 332, 800-805
17. 17 Walter, P. and Johnson, A. E. (1994) Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 10, 87-119
18. 18 Nguyen, M., Millar, D. G., Yong, V. W., Korsmeyer, S. J. and Shore, G. C. (1993) Targeting of Bcl-2 to the mitochondrial outer membrane by a COOH-teminal signal anchor sequence. J. Biol. Chem. 268, 25265-25268
19. 19 Hachiya, N., Mihara, K., Suda, K., Horst, M., Schatz, G. and Lithgow, T. (1995) Reconstitution of the initial steps of mitochondrial protein import. Nature (London) 376, 705-709
20. 20 Komiya, T., Sakaguchi, M. and Mihara, K. (1996) Cytoplasmic chaperones determine the targeting pathway of precursor proteins to mitochondria EMBO J. 15, 399-407
21. 21 Schatz, G. (1996) The protein import system of mitochondria. J. Biol. Chem. 271, 31763-31766
22. 22 Trimble, W. S., Cowan, D. M. and Scheller, R. H. (1988) VAMP-1: a synaptic vesicle-associated integral membrane protein. Proc. Natl. Acad. Sci. U.S.A 85, 4538-4542
23. 23 Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn, Cold Spring Harbor Press, Cold Spring Harbor
24. 24 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature (London) 227, 680-685
25. 25 Zhuang, Z. P., Marks, B. and McCauley, R. B. (1992) The insertion of monoamine oxidase A into the outer membrane of rat liver mitochondria. J. Biol. Chem. 267, 591-596
26. 26 Pfanner, N., Muller, H. K., Harmey, M. A. and Neupert, W. (1987) Mitochondrial protein import: involvement of the mature part of a cleavable precursor protein in the binding to receptor sites. EMBO J 6, 3449-3454
27. 27 Eilers, M., Hwang, S. and Schatz, G. (1988) Unfolding and refolding of a purified precursor protein during import into isolated mitochondria EMBO J 7, 1139-1145
28. 28 Janiak, F., Leber, B. and Andrews, D. W. (1994) Assembly of Bcl-2 into microsomal and outer mitochondrial membranes. J. Biol. Chem. 269, 9842-9849
29. 29 Murakami, H., Pain, D. and Blobel, G. (1988) 70-kDa heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. J. Cell Biol. 107, 2051-2057
30. 30 McBride, H. M., Millar, D. G., Li, J. M. and Shore, G. C. (1992) A signal-anchor sequence selective for the mitochondrial outer membrane. J. Cell Biol. 119, 1451-1457
31. 31 Iwahashi, J., Takaichi, S., Mihara, K. and Omura, T. (1994) Reconstitution of import-competent outer membrane vesicles from mammalian mitochondria. J. Biochem. 116, 156-163
32. 32 Schleiff, E., Silvius, J. R. and Shore, G. C. (1999) Direct membrane insertion of voltage-dependent anion-selective channel protein catalyzed by mitochondrial Tom20. J. Cell Biol. 145, 973-978