Histidine-tagged ubiquitin substitutes for wild-type ubiquitin in Saccharomyces cerevisiae and facilitates isolation and identification of in vivo substrates of the ubiquitin pathway

Analytical Biochemistry

Volumn 282, Issue 1, 2000, Pages 54-64

  Ling, Richard ^a   Colón, Elsbeth ^a   Dahmus, Michael E ^a   Callis, Judy ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Histidine tagged ubiquitin; Nickel chelation chromatography; RNA polymerase II large subunit; Ubiquitin

Indexed keywords

AMINO ACIDS; CHELATION; CHROMATOGRAPHY; COMPLEXATION; PURIFICATION; RNA; SUBSTRATES; YEAST;

ISOLATION AND IDENTIFICATION; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE STRAINS; TRANSGENIC ARABIDOPSIS; TRANSGENIC ORGANISMS; TRANSGENIC PLANTS; UBIQUITIN; UBIQUITINATED PROTEINS;

PROTEINS;

HISTIDINE; RNA POLYMERASE II; UBIQUITIN;

ARABIDOPSIS; ARTICLE; CHELATION; CHROMATOGRAPHY; CONJUGATION; COVALENT BOND; GENETIC TRANSFECTION; IMMUNOBLOTTING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; STARVATION; TRANSGENE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; DAUCUS CAROTA; EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0034660355     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4586     Document Type: Article

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