Flow cytometric evaluation of antibiotic effects on viability and mitochondrial function of refrigerated spermatozoa of Nile tilapia

Theriogenology

Volumn 53, Issue 7, 2000, Pages 1489-1499

  Segovia, M ^a   Jenkins, J A ^b   Paniagua Chavez, C ^a   Tiersch, T R ^a  

^a LOUISIANA STATE UNIVERSITY AGRICULTURAL CENTER   (United States)

^b NATIONAL WETLANDS RESEARCH CENTER   (United States)

Author keywords

Antibiotics; Flow cytometry; Motility; Spermatozoa; Tilapia

Indexed keywords

AMPHOTERICIN B; AMPICILLIN; ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; FLUORESCENT DYE; GENTAMICIN; PROPIDIUM IODIDE; RHODAMINE 123; RINGER SOLUTION; STREPTOMYCIN;

ANIMAL; ANIMAL CELL; ANIMAL DISEASE; ARTICLE; BREEDING; CELL SEPARATION; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; FREEZING; MALE; MITOCHONDRION; NONHUMAN; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON DENSITY; SPERMATOZOON MOTILITY; TILAPIA;

ANIMALS; ANTI-BACTERIAL AGENTS; CELL SEPARATION; CELL SURVIVAL; CRYOPRESERVATION; FLOW CYTOMETRY; MALE; MITOCHONDRIA; SEMEN PRESERVATION; SPERMATOZOA; TILAPIA;

EID: 0034655670     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0093-691X(00)00291-0     Document Type: Article

References (24)

1. Auger J, Ronot X, Dadoune JP. Human sperm mitochondrial function related to motility, a flow and image cytometric assessment. J Androl 1989;10:439-448.
2. Billard R, Cosson J, Perchec G, Linhart O. Biology of sperm and artificial reproduction in carp. Aquaculture 1995;129:95-112.
3. Cotell E, Lennon B, McMorrow J, Barry-Kinsella C, Harrison RF. Processing of semen in an antibiotic-rich culture medium to minimize microbial presence during in vitro fertilization. Fertil Steril 1997;67:98-108.
4. Donoghue AM, Garner DL, Donoghue DJ, Johnson LA. Viability assessment of turkey sperm using fluorescent staining and flow cytometry. Poult Sci 1995;74:1191-1200.
5. Ericsson SA, Garner DL, Thomas CA, Downing TW, Marshall CE. Interrelationships among fluorometric analyses of spermatozoal function, classical semen quality parameters and the fertility of frozen-thawed bovine spermatozoa. Theriogenology 1993;39:1009-1024.
6. Evenson DP, Darzynkiewicz Z, Melamed MR. Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 1982;30:279-280.
7. Evenson DP, Parks JE, Kaproth MT, Jost LK. Rapid determination of sperm cell concentration in bovine semen by flow cytometry. J Dairy Sci 1993;76:86-94.
8. Garner DL, Pinkel D, Johnson LA, Pace MM. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analysis. Biol Reprod 1986;34:127-138.
9. Garner DL, Johnson LA, Yue ST, Roth BL, Haugland RP. Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide. J Androl 1994;15:620-629.
10. Graham JK, Kunze E, Hammerstedt RH. Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry. Biol Reprod 1990;43:55-64.
11. Huyser C, Fourie R, Oosthuizen M, Neethling A. Microbial flora in semen during in vitro fertilization. J in Vitro Fertil Embryo Transf 1991;8:260-264.
12. Jenkins JA. Infectious disease and quality assurance considerations for the transfer of cryopreserved fish gametes. In: Tiersch TR, Mazik PM (eds), Cryopreservation of Aquatic Species. Baton Rouge, LA, World Aquaculture Society, 2000;343-363.
13. Jenkins JA, Tiersch TR. A preliminary bacteriological study of refrigerated channel catfish sperm. J World Aquaculture Soc 1997;28:282-288.
14. Johnson LV, Walsh ML, Chen LB. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci 1980;77:990-994.
15. Johnson LV, Walsh ML, Bockus BJ, Chen LB. Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy. J Cell Biol 1981;88:526-535.
16. Kime De. 1998. Disruption of male reproductive function. In: Endocrine Disruption in Fish, University of Sheffield, Kluwer Sheffield UK Academic Publishers, 1998;121-148.
17. McNiven MA, Gallant RK, Richardson GF. In vitro methods of assessing the viability of rainbow trout spermatozoa. Theriogenology 1992;38:679-686.
18. Ogier De Baulny B, Le Vern Y, Kerboeuf D, Maisse G. Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa. Cryobiology 1997;34:141-149.
19. Rana KJ, McAndrew BJ. The viability of cryopreserved tilapia spermatozoa. Aquaculture 1989;76:335-345.
20. Saad A, Billard R, Theron MC, Hollebecq MG. Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture 1988;71:133-150.
21. SAS User's Guide. Statistics. Cary NC: Statistical Analysis System Institute Inc, 1985.
22. Stoss A. Fish gamete preservation and spermatozoan physiology. In: Hoar WS, Randall DJ, Donaldson EM (eds), Fish Physiology, Vol. IX Reproduction, Part B, Behavior and Fertility Control. New York: Academic Press, 1983; 305-350.
23. Stoss J, Refstie T. Short-term storage and cryopreservation of milt from Atlantic salmon and sea trout. Aquaculture 1983;30:229-236.
24. Truscott RB. Factors associated with the determination of antibiotic activity in bovine semen. Can J Comp Med 1983;47:480-483.