Evaluation of fixation methods for ultrastructural study of Caenorhabditis elegans embryos

Microscopy Research and Technique

Volumn 49, Issue 2, 2000, Pages 212-216

  Vancoppenolle, Bart ^a   Claeys, Myriam ^a   Borgonie, Gaetan ^a   Tytgat, Tom ^a   Coomans, August ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Electron microscopy; Embryology; Fixation; Morphology; Nematodes

Indexed keywords

CAENORHABDITIS ELEGANS; CELLULARS; CHITINASES; ELEGANS; EMBRYOLOGY; FIXATION; FIXATION METHODS; NEMATODE; ULTRASTRUCTURAL STUDY; Α-CHYMOTRIPSIN;

HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY;

CHITINASE; CHYMOTRYPSIN A;

ARTICLE; CAENORHABDITIS ELEGANS; CELL MEMBRANE PERMEABILITY; CELL ULTRASTRUCTURE; EGG SHELL; EMBRYO; EMBRYO DEVELOPMENT; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE; TEMPERATURE; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY;

CAENORHABDITIS ELEGANS; NEMATODA;

EID: 0034655558     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0029(20000415)49:2<212::AID-JEMT15>3.0.CO;2-1     Document Type: Article

References (15)

1. Bird AF, Bird J. 1991. The structure of nematodes, 2nd ed. San Diego: Academic Press.
2. Brenner S. 1974. The genetics of Caenorhabiditis elegans. Genetics 77:71-94.
3. Chitwood BG, Chitwood MB. 1974. Introduction to nematology. Baltimore: University Park Press.
4. Cole TS, Schierenberg E. 1986. Laser microbeam-induced fixation for electronmicroscopy: visualisation of transient developmental features in nematode embryos. Experientia 42:1046-1048.
5. Edgar LG. 1995. Blastomere culture and analysis. In: Epstein HF, Shakes DC, editors. Methods in cell biology, vol. 48. Caenorhabditis elegans: modern biological analysis of an organism. San Diego: Academic Press, p 303-321.
6. Edgar LG, McGhee J. 1988. DNA synthesis and the control of embryonic gene expression in C. elegans. Cell 53:589-599.
7. Hall DH. 1995. Electron microscopy and three-dimensional image reconstruction. In: Epstein HF, Snakes DC, editors. Methods in cell biology, vol. 48. Caenorhabditis elegans: modern biological analysis of an organism. San Diego: Academic Press, p 396-436.
8. Hyman AA. 1989. Centrosome movement in the early divisions of Caenorhabditis elegans: a cortical site determining centrosome position. J Cell Biol 109:1185-1193.
9. Krieg C, Cole T, Deppe U, Schierenberg E, Schmitt D, Yoder B, von Ehrenstein G. 1978. The cellular anatomy of embryos of the nematode Caenorhabditis elegans. Dev Biol 65:193-215.
10. Priess JR, Hirsh DI. 1986. Caenorhabditis elegans morphogenesis: the role of the cytoskeleton in elongation of the embryo. Dev Biol 117: 156-173.
11. Schierenberg E. 1989. Cytoplasmic determination and distribution of developmental potential in the embryo of Caenorhabditis elegans. BioEssays 10:99-104.
12. Schierenberg E, Cole T, Carlson C, Sidio W. 1986. Computer-aided three-dimensional reconstruction of nematode embryos from EM serial sections. Exp Cell Res 166:247-252.
13. Sulston JE, Schierenberg E, White JG, Thomson JN. 1983. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev Biol 100:64-119.
14. Weiser MM, Walters JRF, Wilson JR. 1988. Intestinal cell membranes. Int Rev Cytol 101:1-49.
15. Wolf N, Priess J, Hirsh D. 1983. Segregation of germline granules in early embryos of Caenorhabditis elegans: an electron microscopic analysis. J Embryol Exp Morphol 73:297-306.