A high-throughput screening method for the determination of aqueous drug solubility using laser nephelometry in microtiter plates

Analytical Chemistry

Volumn 72, Issue 8, 2000, Pages 1781-1787

  Bevan, Christopher D ^a   Lloyd, Richard S ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ONDANSETRON;

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; DRUG SCREENING; DRUG SOLUBILITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; LASER NEPHELOMETRY; REPRODUCIBILITY; VALIDATION PROCESS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; NEPHELOMETRY AND TURBIDIMETRY; PHARMACEUTICAL PREPARATIONS; SOLUBILITY; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0034655372     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac9912247     Document Type: Article

References (7)

1. Vogel, A. J. A TextBook of Quantitative Inorganic Analysis, 5th ed.; Longman: London, 1998.
2. Lipinski, C. A.; Lombardo, F.; Dominy, B. W.; Feeney, P. J. Adv. Drug Delivery Rev. 1997, 23, 3-25.
3. Lipinski, C. A. IBC 6th Annual Conference on Rational Drug Design, 1997.
4. Quartermain, C. P.; Bonham, N. M.; Irwin, A. K. Eur. Pharm. Rev. 1998, 18 (4), 27-32.
5. Furer, R.; Geiger, M. Pestic. Sci. 1977, 8, 337-344.
6. Goodwin, J.; Merrill, S.; Asa, D. A New Rapid Technique for Sensitive Solubility Measurements: a Flow Cytometric Approach. A poster presented at the 5th Annual Society for Biomolecular Screening (SBS) Conference, Edinburgh, September, 13-16, 1999.
7. Precipitation from DMSO method: Drug solubility determination is based on the dilution of a sample supplied as a DMSO solution (10 mM) diluted with buffer. The measurement of concentration after filtration is achieved by comparison of the UV absorbance of the saturated solution with that of a known standard following an HPLC separation. The standard solution is prepared by dissolving a known weight of compound in a suitable solvent such as methanol or DMSO.