Functional analysis of heterologous holin proteins in a λΔS genetic background

FEMS Microbiology Letters

Volumn 184, Issue 2, 2000, Pages 179-186

  Vukov, Nataša ^a   Scherer, Siegfried ^a   Hibbert, Edward ^a   Loessner, Martin J ^a  

^a Forschungszentrum Milch Lebensm W   (Germany)

Author keywords

Bacteriophage; Endolysin; Holin; Lysis; Membrane protein

Indexed keywords

HOLIN PROTEIN; HYDROLASE; MEMBRANE PROTEIN; PEPTIDOGLYCAN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE LAMBDA; CONTROLLED STUDY; CYTOLYSIS; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE T7; BASE SEQUENCE; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; LYSOGENY; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; VIRAL PROTEINS;

BACTERIOPHAGE LAMBDA; COLI PHAGE; ESCHERICHIA COLI; LISTERIA; STAPHYLOCOCCUS AUREUS;

EID: 0034654341     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00041-0     Document Type: Article

References (21)

1. Young R. Bacteriophage lysis: Mechanisms and regulation. Microbiol. Rev. 56:1992;430-481
2. Young R., Bläsi U. Holins: form and function in bacteriophage lysis. FEMS Microbiol. Rev. 17:1995;191-205
3. Loessner M.J., Gaeng S., Scherer S. Evidence for a holin-like protein gene fully embedded out of frame in the endolysin gene of Staphylococcus aureus bacteriophage 187. J. Bacteriol. 181:1999;4452-4460
4. Lu M.J., Henning U. Lysis protein T of bacteriophage T4. Mol. Gen. Genet. 235:1992;253-258
5. Bläsi U., Young R. Two beginnings for a single purpose: the dual-start holins in the regulation of phage lysis. Mol. Microbiol. 21:1996;675-682
6. Johnson-Boaz R., Chang C.-Y., Young R. A dominant mutation in the bacteriophage λS gene causes premature lysis and an absolute defective plating phenotype. J. Bacteriol. 13:1994;495-504
7. Bläsi U., Nam K., Hartz D., Gold L., Young R. Dual translation initiation sites control function of the λS gene. EMBO J. 8:1989;3501-3510
8. Berkmen M., Benedik J.M., Bläsi U. The Serratia marcescens NucE protein functions as a holin in Escherichia coli. J. Bacteriol. 179:1997;6522-6524
9. Gasson M.J., Anderson P.H. High copy number plasmid vector for use in lactic streptococci. FEMS Microbiol. Lett. 30:1985;193-196
10. Ackerman, H.-W., DuBow, M.S. (1987) In: Viruses of Procaryotes, Vol. II: Natural Group of Bacteriophages. CRC Press. Boca Raton, FL
11. Raab R., Neal G., Sohaskey C., Smith J., Young R. Dominance in λS mutation and evidence for translational control. J. Mol. Biol. 199:1998;95-105
12. Sambrook, J., Maniatis, T., Fritsch, E. F. (1989) Molecular cloning: a laboratory manual, 2nd edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
13. Loessner M.J., Wendlinger G., Scherer S. Heterogeneous endolysins in Listeria monocytogenes bacteriophages: a new class of enzymes and evidence for conserved holin genes within the siphoviral lysis cassettes. Mol. Microbiol. 16:1995;1231-1241
14. Loessner J.M., Gaeng S., Wendlinger G., Maier K.S., Scherer S. The two-component lysis system of Staphylococcus aureus bacteriophage Twort: a large TTG-start and an associated amidase endolysin. FEMS Microbiol. Lett. 162:1998;265-274
15. Bläsi U., Chang Ch.-Yu., Zagotta T.M., Nam K., Young R. The lethal λS encodes its own inhibitor. EMBO J. 9:1990;981-989
16. Steiner M., Bläsi U. Charged amino-terminal amino acids affect the lethal capacity of λ lysis proteins S107 and S105. Mol. Microbiol. 8:1993;525-533
17. Graschopf A., Bläsi U. Molecular function of the dual-start motif in the λS holin. Mol. Microbiol. 33:1999;569-582
18. Barenboim M., Chang C.-Y., dib Hajj F., Young R. Characterization of the dual start motif of a class II holin gene. Mol. Microbiol. 32:1999;715-727
19. Tedin K., Resch A., Steiner M., Bläsi U. Dual translational start motiv evolutionarily conserved in the holin gene of Bacillus subtilis phage φ29. Virology. 206:1995;479-484
20. Rietsch A., Freisl P., Graschopf A., Bläsi U. The hydrophilic C-terminal part of the λS holin is non-essential for intermolecular interaction. FEMS Microbiol. Lett. 162:1997;265-274
21. Bläsi U., Freisl P., Chang C.-Y., Zhang N., Young R. The C-Terminal sequence of the λ Holin constitutes a cytoplasmatic regulatory domain. J. Bacteriol. 181:1999;2922-2929