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Volumn 122, Issue 10, 2000, Pages 2389-2390

The structure of the metal-binding motif GMTCAAC is similar in an 18- residue linear peptide and the mercury binding protein MerP [10]

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; MERCURY; METAL; PEPTIDE;

EID: 0034654122     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992908z     Document Type: Letter
Times cited : (50)

References (33)
  • 1
    • 0002930694 scopus 로고
    • Stillman, M. J., Shaw, C. F., Suzuki, K. T., Eds.; VCH: New York
    • Stillman, M. J. In Metallothioneins; Stillman, M. J., Shaw, C. F., Suzuki, K. T., Eds.; VCH: New York, 1992; pp 55-127.
    • (1992) Metallothioneins , pp. 55-127
    • Stillman, M.J.1
  • 13
    • 0342721830 scopus 로고    scopus 로고
    • note
    • 2O, 20 mM phosphate buffer (pH ∼6.5), 1 mM DTT for a final concentration of 1.5 mM. All of the experiments were performed on a Bruker DMX 500 spectrometer.
  • 14
    • 0003769049 scopus 로고    scopus 로고
    • Structure calculations were performed using the computer program XPLOR version 3.1 and a hybrid distance geometry/simulated annealing protocol. Two independent calculations were performed in the presence and in the absence of metal. A total of 77 NOEs were assigned and classified as strong, medium, and weak corresponding to distance restraints of 1.9-2.7, 1.9-3.3, and 1.9-5.0 Å, respectively. Three dihedral angles constraints were used in the calculations. Both calculations converged in the same folding. No NOE violations greater than 0.35 Å or dihedral angle violations greater than 5° were found. The 10 lowest-energy structures were used for further analysis. See: Brunger, A. T. In X-PLOR, version 3.1; A System for X-ray Crystallography and NMR; Yale University Press: New Haven, CT, 1992; Nilges, M.; Clore, M. Gronenborn A. FEBS Lett. 1988, 239, 129-136.
    • X-PLOR, Version 3.1
    • Brunger, A.T.1
  • 15
    • 0037877123 scopus 로고
    • Yale University Press: New Haven, CT
    • Structure calculations were performed using the computer program XPLOR version 3.1 and a hybrid distance geometry/simulated annealing protocol. Two independent calculations were performed in the presence and in the absence of metal. A total of 77 NOEs were assigned and classified as strong, medium, and weak corresponding to distance restraints of 1.9-2.7, 1.9-3.3, and 1.9-5.0 Å, respectively. Three dihedral angles constraints were used in the calculations. Both calculations converged in the same folding. No NOE violations greater than 0.35 Å or dihedral angle violations greater than 5° were found. The 10 lowest-energy structures were used for further analysis. See: Brunger, A. T. In X-PLOR, version 3.1; A System for X-ray Crystallography and NMR; Yale University Press: New Haven, CT, 1992; Nilges, M.; Clore, M. Gronenborn A. FEBS Lett. 1988, 239, 129-136.
    • (1992) A System for X-ray Crystallography and NMR
  • 16
    • 0023732144 scopus 로고
    • Structure calculations were performed using the computer program XPLOR version 3.1 and a hybrid distance geometry/simulated annealing protocol. Two independent calculations were performed in the presence and in the absence of metal. A total of 77 NOEs were assigned and classified as strong, medium, and weak corresponding to distance restraints of 1.9-2.7, 1.9-3.3, and 1.9-5.0 Å, respectively. Three dihedral angles constraints were used in the calculations. Both calculations converged in the same folding. No NOE violations greater than 0.35 Å or dihedral angle violations greater than 5° were found. The 10 lowest-energy structures were used for further analysis. See: Brunger, A. T. In X-PLOR, version 3.1; A System for X-ray Crystallography and NMR; Yale University Press: New Haven, CT, 1992; Nilges, M.; Clore, M. Gronenborn A. FEBS Lett. 1988, 239, 129-136.
    • (1988) FEBS Lett. , vol.239 , pp. 129-136
    • Nilges, M.1    Clore, M.2    Gronenborn, A.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.