Selective organic precipitation/extraction of released N-glycans following large-scale enzymatic deglycosylation of glycoproteins

Analytical Biochemistry

Volumn 278, Issue 2, 2000, Pages 111-122

  Verostek, Mary Frances ^a   Lubowski, Catherine ^a   Trimble, Robert B ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Endo and peptide N glycosidases; Glycoproteins; N and O linked glycans; Oligosaccharide isolation; Solvent precipitation

Indexed keywords

ACETONE; DESALINATION; GLYCOPROTEINS; MAMMALS; OLIGOSACCHARIDES; PEPTIDES; POLYSACCHARIDES; YEAST;

AQUEOUS METHANOL; DEGLYCOSYLATION; EQUIPMENT REQUIREMENTS; GLYCOSIDASES; N-ACETYLGLUCOSAMINIDASE; O-LINKED; PREPARATIVE CHROMATOGRAPHY; SOLVENT PRECIPITATION;

CHROMATOGRAPHY;

ACETONE; BETA FRUCTOFURANOSIDASE; FETUIN; GLYCAN; GLYCOPROTEIN; MANNOSE; METHANOL; N ACETYL BETA GLUCOSAMINIDASE; OLIGOSACCHARIDE; OVALBUMIN; RIBONUCLEASE; THYROGLOBULIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; DEGLYCOSYLATION; EXTRACTION; PRECIPITATION; PRIORITY JOURNAL;

BOVINAE; PICHIA PASTORIS;

EID: 0034651589     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4433     Document Type: Article

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