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Volumn 288, Issue 5468, 2000, Pages 1051-1053

Influenza B virus in seals

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

EID: 0034640380     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5468.1051     Document Type: Article
Times cited : (298)

References (30)
  • 1
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    • B. R. Murphy and R. G. Webster, in Virology, B. N. Fields et al., Eds. (Raven, New York, 1990).
    • (1990) Virology
    • Murphy, B.R.1    Webster, R.G.2
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  • 11
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    • C. D. Harvell et al., Science 285, 1505 (1999).
    • (1999) Science , vol.285 , pp. 1505
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    • J. R. Geraci et al., Science 215, 1129 (1982).
    • (1982) Science , vol.215 , pp. 1129
    • Geraci, J.R.1
  • 18
    • 0343937882 scopus 로고    scopus 로고
    • note
    • 2, 1 mM dNTP, and 400 nM each of primer. Cycling parameters were 30 min at 42°C, 4 min at 95°C, 1 min at 45°C, and 3 min at 72°C once; and then 1 min at 95°C, 1 min at 45°C, and 3 min at 72°C, repeated 39 times. PCR fragments were sequenced with a DYEnamic ET terminator cycle sequencing premix kit (Amersham) on an ABI-373A apparatus (Perkin Elmer).
  • 19
    • 0342631836 scopus 로고    scopus 로고
    • note
    • 50 of influenza virus B/Seal/Netherlands/1/99 in DMEM supplemented with 4% bovine serum albumin, 1% L-glutamine, penicillin, and streptomycin. Influenza B virus infection was detected by immunofluorescence with influenza B NP-specific antibodies, which were labeled with fluorescein isothiocyanate (IMAGEN Influenza A+B, DAKO Diagnostics) after 24 hours. Cytopathic changes and HA activity (titer = 32) were detected in the culture cell after 48 hours.
  • 21
    • 0342631837 scopus 로고    scopus 로고
    • note
    • Antibody titers were determined with a recombinant fusion protein between maltose-binding protein (MBP) and NP or with HA/NA proteins purified from virions (both proteins were derived from B/Harbin/7/ 94). IgG antibody levels to HA/NA and NP proteins were determined by an indirect ELISA with antigen-coated plates and peroxidase-labeled protein A for detection. IgM antibody levels to NP were determined by means of an antibody-capture ELISA with goat anti-dog IgM-coated plates and peroxidase-labeled MBP-NP antigen for detection The goat antidog IgM antibody preparation specifically captures seal IgM, as was shown in routine serological tests for PDV and PHV.
  • 22
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    • Blackwell Scientific, Oxford, ed. 8
    • I. M. Roitt, Essential Immunology (Blackwell Scientific, Oxford, ed. 8, 1994).
    • (1994) Essential Immunology
    • Roitt, I.M.1
  • 23
    • 0342631528 scopus 로고    scopus 로고
    • The sequences of influenza B virus HA and NS genes are available at www.flu.lanl.gov/.
  • 24
    • 0343502028 scopus 로고    scopus 로고
    • note
    • Analysis of the HA1 sequences from human influenza B virus strains that are available from the influenza sequence database showed that between 1991 and 1999, the proportion of strains with >98% nucleotide identity to B/Seal/Netherlands/1/99 was 0, 17, 27, 46, 62, 29, 0, 0, and 0% of all epidemic strains for each successive year, respectively.
  • 29
    • 0343502023 scopus 로고    scopus 로고
    • note
    • HA1 sequences were amplified with M13-tagged primers P1 (5′-29M13-GCA TTT TCT AAT ATC CAC AA-3′) and P4 (5′-21M13-TTT GGG AAG CCA CCA ATC TG-3′) or primers P3 (5′-29M13-CCT ATA ATG CAC GAC AGA AC-3′) and P6 (5′-21M13-AAA CCA GCA ATA GCT CCG AA-3′), followed by sequencing with primers 29M13 (5′-CAG GAA ACA GCT ATG ACC-3′) and 21M13 (5′-TGT AAA ACG ACG GCC AGT-3′) using a DYEnamic ET terminator cycle sequencing premix kit (Amersham) and an ABI-373A sequencing apparatus (Perkin Elmer).
  • 30
    • 0343502024 scopus 로고    scopus 로고
    • note
    • We are grateful to L. van der Kemp, C. de Mutsert, and M. van der Bildt for technical assistance; J. Habova for electron microscopy; Solvay Pharmaceuticals, Weesp, the Netherlands, for providing HA/NA proteins from B/Harbin/7/94; and the SRRC staff for taking care of and samples from seals. R. F. is a fellow of the Royal Netherlands Academy of Arts and Sciences.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.