Microtubules switch occasionally into unfavorable configurations during elongation

Journal of Molecular Biology

Volumn 298, Issue 4, 2000, Pages 663-676

  Chrétien, Denis ^a,b   Fuller, Stephen D ^b,c  

^a UPRES A CNRS 6026   (France)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Cryo electron microscopy; Image analysis; Microtubule stability; Microtubule structure

Indexed keywords

TUBULIN;

ARTICLE; CATTLE; CHEMICAL MODIFICATION; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; IMAGE ANALYSIS; MICROTUBULE ASSEMBLY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; STOCHASTIC MODEL; STRUCTURE ANALYSIS;

BOS TAURUS;

EID: 0034640129     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3696     Document Type: Article

References (56)

1. Arrangement of subunits in flagellar microtubules
2. XMAP310: A Xenopus rescue-promoting factor localized to the mitotic spindle
3. Low resolution structure of microtubules in solution. Synchrotron X-ray scattering and electron microscopy of taxol-induced microtubules assembled from purified tubulin in comparison with glycerol and MAP-induced microtubules
4. How does taxol stabilize microtubules?
5. Three-dimensional structure of functional motor proteins on microtubules
6. The in vitro assembly of flagellar outer doublet tubulin
7. Structural and chemical characterization of isolated centrosomes
8. Electron microscope studies of pH effects on assembly of tubulin free of associated proteins
9. Role of nucleotide hydrolysis in the dynamics of actin filaments and microtubules
10. New data on the microtubule surface lattice
11. Lattice defects in microtubules: Protofilament numbers vary within individual microtubules
12. Structure of growing microtubule ends: Two-dimensional sheets close into tubes at variable rates
13. Determination of microtubule polarity by cryo-electron microscopy
14. Reconstruction of the centrosome cycle from cryo-electron micrographs
15. Limited flexibility of the inter-protofilament bonds in microtubules
16. Microtubule's conformational cap
17. Microtubule polymerization dynamics
18. Kin I kinesins are microtubule-destabilizing enzymes
19. Mechanism of microtubule assembly. Changes in polymer structure and organization during assembly of sea urchin egg tubulin
20. Microtubule surface lattice and subunit structure and observations on reassembly
21. Microtubule dynamic instability and GTP hydrolysis
22. Influence of the centrosome on the structure of nucleated microtubules
23. Flexural rigidity of microtubules measured with the use of optical tweezers
24. Three-dimensional cryoelectron microscopy of 16-protofilament microtubules: Structure, polarity, and interaction with motor proteins
25. Structural changes accompanying GTP hydrolysis in microtubules: Information from a slowly hydrolyseable analogue guanylyl-(α-β)-methylene-diphosphonate
26. Modeling elastic properties of microtubule tips and wall
27. Direct visualization of the microtubule lattice seam both in vitro and in vivo
28. Three-dimensional structure of the kinesin head-microtubule complex
29. Quantitative electron microscopy of microtubule assembly in vitro
30. Arrangement of subunits in microtubules with 14 protofilaments
31. Reassembly of flagellar B(αβ) tubulin into singlet microtubules: Consequences for cytoplasmic microtubule structure and assembly
32. Approaching microtubule structure with the scanning tunneling microscope
33. Unstained microtubules studied by cryo-electron microscopy: Substructure, supertwist and disassembly
34. On the surface lattice of microtubules: Helix starts, protofilament number, seam, and handedness
35. Microtubule dynamics and microtubule caps: A time-resolved cryo-electron microscopy study
36. Cold depolymerization of microtubules to double rings: Geometric stabilization of assemblies
37. Tomography without tilt: Three-dimensional imaging of microtubule/motor complexes
38. Rigidity of microtubules is increased by stabilizing agents
39. Dynamic instability of microtubule growth
40. Structure of αβ tubulin dimer by electron crystallography
41. High-resolution model of the microtubule
42. Microtubule bending and breaking in loiving fibroblast cells
43. Alterations in number of protofilaments in microtubules assembled in-vitro
44. Kinesin follows the microtubule's protofilament axis
45. The structure of microtubule ends during the elongation and shorterring phases of dynamic instability examined by negative stain electron microscopy
46. The anatomy of flagellar microtubules: Polarity, seam, junctions, and lattice
47. Relationship between moire patterns, tubulin shape and microtubule polarity
48. Three-dimensional structure of ncd-decorated microtubules obtained by a back-projection method
49. Three different approaches for calculating the three-dimensional structure of microtubules decorated with kinesin motor domains
50. Microtubules: Evidence for 13 protofilaments
51. XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover
52. Analysis of microtubule rigidity using hydrodynamic flow and thermal fluctuations
53. Cryoelectron microscopy of microtubules
54. Characterization of microtubule protofilament numbers; how does the surface lattice accommodate?